Abstract
Objective: Evaluation of serum beta-2-microglobulin (β2M) in an automated analyzer. Design and methods: The DakoCytomation β2M kit is an antibody based reagent intended for quantitative determination of β2M in serum and plasma by rate nephelometry. Results: The limit of blank is 0.16 mg/L. The method is linear up to 17.9 mg/L. The imprecision ranged from 2.1% to 7.9% at the concentrations of 1.77 and 7.19 mg/L, respectively. Method comparison yielded slope = 1.009, r = 0.998. No interference was observed from hemolytic or icteric specimens. Reference interval of a healthy population was 1.13 mg/L to 3.04 mg/L. Conclusion: The DakoCytomation reagent is acceptable to measure serum β2M.
Original language | English (US) |
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Pages (from-to) | 1577-1581 |
Number of pages | 5 |
Journal | Clinical Biochemistry |
Volume | 42 |
Issue number | 15 |
DOIs | |
State | Published - Oct 2009 |
Externally published | Yes |
Keywords
- Beta-2-microglobulin
- DakoCytomation
- Rate nephelometry
- Tumor marker
ASJC Scopus subject areas
- Clinical Biochemistry