Perchlorate induces hermaphroditism in threespine sticklebacks

Richard R. Bernhardt, Frank A. Von Hippel, William A. Cresko

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Recently, concern regarding perchlorate contamination has arisen in many contexts. Perchlorate has many military, commercial, and domestic applications, and it has been found in milk, drinking and irrigation water, and produce. Perchlorate is harmful at low levels, yet it remains unregulated in the United States while the U.S. Environmental Protection Agency attempts to establish acceptable exposure levels. The present study investigated potential reproductive effects on vertebrates using a model fish species, the threespine stickleback (Gasterosteus aculeatus). Sticklebacks were raised from syngamy through sexual maturity in untreated water and in three target concentrations of sodium perchlorate-treated water. Perchlorate was found to interfere with the expression of nuptial coloration, courtship behavior, and normal sexual development. Genetic testing revealed that some females were masculinized to the extent that they produced both sperm and eggs, and histological analysis showed that these individuals had intersexual gonads (ovotestes) containing both oocytes and cells undergoing spermatogenesis. In vitro fertilizations revealed that those gametes were capable of self- and cross-fertilization. However, crosses using sperm derived from genetic females died either during the blastula phase or near the onset of organogenesis. Sperm derived from genetic males produced viable fry when crossed with eggs derived from genetic females from all treatments. To our knowledge, the present study provides the first evidence that perchlorate produces androgenic effects and is capable of inducing functional hermaphroditism in a nonhermaphroditic vertebrate.

Original languageEnglish (US)
Pages (from-to)2087-2096
Number of pages10
JournalEnvironmental Toxicology and Chemistry
Volume25
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

Keywords

  • Endocrine disruption
  • Gasterosteus aculeatus
  • Hermaphroditism
  • Perchlorate
  • Threespine stickleback

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

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