TY - JOUR
T1 - Per os challenge of Litopenaeus vannamei postlarvae and Farfantepenaeus duorarum juveniles with six geographic isolates of white spot syndrome virus
AU - Wang, Qiong
AU - White, Brenda L.
AU - Redman, Rita M.
AU - Lightner, Donald V.
N1 - Funding Information:
This research was funded by the Gulf Coast Research laboratory Consortium Marine Shrimp Farming Program, CSRS, USDA under Grant No. 88-388083320, the National Sea Grant Program, USDC under the Grant No. NA56RG0617, USDC under Grant No. NA56FD0621, and a grant from the National Fishery institute. The original isolates of WSSV were kindly provided by Dr. Paul Frelier (Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station, TX), Dr. Craig Browdy (South Carolina Department of Natural Resources, Waddell Mariculture Center, Bluffton, South Carolina), Dr. Laura K. Richman (Department of Pathology, National Zoological Park, Washington DC), Dr. Eileen Reddington (Diagxotics), Dr. Ramana Murty (Andhra University, Visakhapatnam, India), and Mr. Jie Huang (Yellow Sea Fishery Research Institute, Qingdao, Shangdong, China). Mr. Jim Sweeny and Dr. Will Carr (The Oceanic Institute, Makapuu Point, Hawaii) provided the SPF Litopenaeus vannamei and Dr. David Vaughan (Harbor Branch Oceanographic Institute, Fort Pierce, FL) provided the stocks of Farfantepenaeus duorarum. Jeffery R. Garza is thanked for assistance with computer graphics.
PY - 1999/1/15
Y1 - 1999/1/15
N2 - White spot syndrome virus (WSSV) is one of the most important pathogens of penaeid shrimp. It is widely distributed in most Asian countries where penaeid shrimp are cultured, as well as in the Gulf of Mexico and SE USA. The virulence of six geographic isolates of WSSV was compared using Litopenaeus vannamei postlarvae and Farfantepenaeus duorarum juveniles. The six geographic isolates of WSSV originated from China, India, Thailand, Texas, South Carolina, as well as from crayfish maintained at the USA National Zoo. For challenge studies, virus infected tissues were given per os to L. vannamei postlarvae and Fa. duorarum juveniles. Resultant WSSV infections were confirmed by histological examination. The cumulative mortality of L. vannamei postlarvae reached 100% after challenge with each of the six geographic isolates of WSSV. However, the Texas isolate caused mortalities more rapidly than did the other shrimp isolates; the crayfish WSSV isolate was the slowest. In marked contrast, cumulative mortalities of juvenile Fa. duorarum reached only 35-60%, and varied among the geographic isolates of WSSV. Interestingly, in Fa. duorarum, the Texas WSSV isolate was also the most virulent, while the crayfish WSSV was the least virulent. The findings suggest that slight differences in virulence exist among geographic isolates of WSSV, and that susceptibility may vary with species and lifestages of the host.
AB - White spot syndrome virus (WSSV) is one of the most important pathogens of penaeid shrimp. It is widely distributed in most Asian countries where penaeid shrimp are cultured, as well as in the Gulf of Mexico and SE USA. The virulence of six geographic isolates of WSSV was compared using Litopenaeus vannamei postlarvae and Farfantepenaeus duorarum juveniles. The six geographic isolates of WSSV originated from China, India, Thailand, Texas, South Carolina, as well as from crayfish maintained at the USA National Zoo. For challenge studies, virus infected tissues were given per os to L. vannamei postlarvae and Fa. duorarum juveniles. Resultant WSSV infections were confirmed by histological examination. The cumulative mortality of L. vannamei postlarvae reached 100% after challenge with each of the six geographic isolates of WSSV. However, the Texas isolate caused mortalities more rapidly than did the other shrimp isolates; the crayfish WSSV isolate was the slowest. In marked contrast, cumulative mortalities of juvenile Fa. duorarum reached only 35-60%, and varied among the geographic isolates of WSSV. Interestingly, in Fa. duorarum, the Texas WSSV isolate was also the most virulent, while the crayfish WSSV was the least virulent. The findings suggest that slight differences in virulence exist among geographic isolates of WSSV, and that susceptibility may vary with species and lifestages of the host.
KW - Geographic isolates
KW - Penaeid shrimp
KW - Susceptibility
KW - Virulence
KW - WSSV
KW - White spot syndrome virus
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U2 - 10.1016/S0044-8486(98)00425-6
DO - 10.1016/S0044-8486(98)00425-6
M3 - Article
AN - SCOPUS:0033555341
SN - 0044-8486
VL - 170
SP - 179
EP - 194
JO - Aquaculture
JF - Aquaculture
IS - 3-4
ER -