TY - JOUR
T1 - Pemphigus vulgaris antigen mRNA quantification for the staging of sentinel lymph nodes in head and neck cancer
AU - Solassol, J.
AU - Burcia, V.
AU - Costes, V.
AU - Lacombe, J.
AU - Mange, A.
AU - Barbotte, E.
AU - De Verbizier, D.
AU - Cartier, C.
AU - Makeieff, M.
AU - Crampette, L.
AU - Boulle, N.
AU - Maudelonde, T.
AU - Guerrier, B.
AU - Garrel, R.
N1 - Funding Information:
This study was supported by grants from the Société Franc¸aise de Carcinologie Cervico-Faciale 2007 and CHRU Montpellier AOI 2007.
PY - 2010/1
Y1 - 2010/1
N2 - Background: Molecular diagnosis has been proposed to enhance the intra-operative diagnosis of sentinel lymph node (SLN) invasion in head and neck squamous cell carcinoma (HNSCC). Although cytokeratin (CK) mRNA quantification with real-time reverse transcriptase-PCR (QRT-PCR) has produced encouraging results, the more discriminating markers remain to be identified.Methods: Pemphigus vulgaris antigen (PVA), squamous cell carcinoma antigen (SCCA), and CK17 mRNA were quantified using QRT-PCR, and the results were compared with an extensive histopathological examination of the entire SLNs on 78 SLNs harvested from 22 patients with HNSCC.Results: SCCA and CK17 quantification showed significantly higher mRNA values for macrometastases (MAs) than for either negative or isolated tumour cell (ITC) SLNs (P<0.01). Pemphigus vulgaris antigen allowed the discrimination of all MAs and micrometastases from both negative and ITC SLNs (P<0.001). For the neck staging of patients, considering metastatic vs non-metastatic status, receiver-operating characteristic curve analysis found areas under the curve of 93.8, 97.9, and 100% for CK17, SCCA, and PVA, respectively. With PVA, a cutoff value of 562 copies per 100 ng of cDNA permitted the correct distinction between patients with positive as opposed to negative neck nodes in all cases.Conclusion: PVA seems to be a highly promising marker for accurate intra-operative SLN staging in HNSCC by QRT-PCR.
AB - Background: Molecular diagnosis has been proposed to enhance the intra-operative diagnosis of sentinel lymph node (SLN) invasion in head and neck squamous cell carcinoma (HNSCC). Although cytokeratin (CK) mRNA quantification with real-time reverse transcriptase-PCR (QRT-PCR) has produced encouraging results, the more discriminating markers remain to be identified.Methods: Pemphigus vulgaris antigen (PVA), squamous cell carcinoma antigen (SCCA), and CK17 mRNA were quantified using QRT-PCR, and the results were compared with an extensive histopathological examination of the entire SLNs on 78 SLNs harvested from 22 patients with HNSCC.Results: SCCA and CK17 quantification showed significantly higher mRNA values for macrometastases (MAs) than for either negative or isolated tumour cell (ITC) SLNs (P<0.01). Pemphigus vulgaris antigen allowed the discrimination of all MAs and micrometastases from both negative and ITC SLNs (P<0.001). For the neck staging of patients, considering metastatic vs non-metastatic status, receiver-operating characteristic curve analysis found areas under the curve of 93.8, 97.9, and 100% for CK17, SCCA, and PVA, respectively. With PVA, a cutoff value of 562 copies per 100 ng of cDNA permitted the correct distinction between patients with positive as opposed to negative neck nodes in all cases.Conclusion: PVA seems to be a highly promising marker for accurate intra-operative SLN staging in HNSCC by QRT-PCR.
KW - Diagnostic accuracy
KW - Head and neck cancer
KW - Immunohistochemistry
KW - Pemphigus vulgaris antigen
KW - Sentinel lymph nodes
UR - https://www.scopus.com/pages/publications/74249114392
UR - https://www.scopus.com/pages/publications/74249114392#tab=citedBy
U2 - 10.1038/sj.bjc.6605470
DO - 10.1038/sj.bjc.6605470
M3 - Article
C2 - 19997107
AN - SCOPUS:74249114392
SN - 0007-0920
VL - 102
SP - 181
EP - 187
JO - British journal of cancer
JF - British journal of cancer
IS - 1
ER -