PBDX is the XG blood group gene

Nathan A. Ellis; Patricia Tippett; Alison Petty; Marion Reid; Polly A. Weller; Tian Z. Ye; James German; Peter N. Goodfellow; Stephen Thomas; George Banting

doi:10.1038/ng1194-285

PBDX is the XG blood group gene

Nathan A. Ellis
, Patricia Tippett
, Alison Petty
, Marion Reid
, Polly A. Weller
, Tian Z. Ye
, James German
, Peter N. Goodfellow
, Stephen Thomas
, George Banting

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

We have identified the Xg^a antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xg^a. Therefore, by its identity with PBDX, Xg^a is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

Original language	English (US)
Pages (from-to)	285-290
Number of pages	6
Journal	Nature Genetics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1038/ng1194-285
State	Published - Nov 1994
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1038/ng1194-285

Cite this

@article{2e1043ebfb204471ad89fc4555380c70,

title = "PBDX is the XG blood group gene",

abstract = "We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48\% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.",

author = "Ellis, \{Nathan A.\} and Patricia Tippett and Alison Petty and Marion Reid and Weller, \{Polly A.\} and Ye, \{Tian Z.\} and James German and Goodfellow, \{Peter N.\} and Stephen Thomas and George Banting",

year = "1994",

month = nov,

doi = "10.1038/ng1194-285",

language = "English (US)",

volume = "8",

pages = "285--290",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature Research",

number = "3",

}

TY - JOUR

T1 - PBDX is the XG blood group gene

AU - Ellis, Nathan A.

AU - Tippett, Patricia

AU - Petty, Alison

AU - Reid, Marion

AU - Weller, Polly A.

AU - Ye, Tian Z.

AU - German, James

AU - Goodfellow, Peter N.

AU - Thomas, Stephen

AU - Banting, George

PY - 1994/11

Y1 - 1994/11

N2 - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

AB - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

UR - https://www.scopus.com/pages/publications/0027996829

UR - https://www.scopus.com/pages/publications/0027996829#tab=citedBy

U2 - 10.1038/ng1194-285

DO - 10.1038/ng1194-285

M3 - Article

C2 - 7533029

AN - SCOPUS:0027996829

SN - 1061-4036

VL - 8

SP - 285

EP - 290

JO - Nature Genetics

JF - Nature Genetics

IS - 3

ER -

PBDX is the XG blood group gene

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this