PBDX is the XG blood group gene

Nathan A. Ellis; Patricia Tippett; Alison Petty; Marion Reid; Polly A. Weller; Tian Z. Ye; James German; Peter N. Goodfellow; Stephen Thomas; George Banting

doi:10.1038/ng1194-285

PBDX is the XG blood group gene

Nathan A. Ellis, Patricia Tippett, Alison Petty, Marion Reid, Polly A. Weller, Tian Z. Ye, James German, Peter N. Goodfellow, Stephen Thomas, George Banting

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

We have identified the Xg^a antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xg^a. Therefore, by its identity with PBDX, Xg^a is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

Original language	English (US)
Pages (from-to)	285-290
Number of pages	6
Journal	Nature Genetics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1038/ng1194-285
State	Published - Nov 1994
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1038/ng1194-285

Cite this

@article{2e1043ebfb204471ad89fc4555380c70,

title = "PBDX is the XG blood group gene",

abstract = "We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.",

author = "Ellis, {Nathan A.} and Patricia Tippett and Alison Petty and Marion Reid and Weller, {Polly A.} and Ye, {Tian Z.} and James German and Goodfellow, {Peter N.} and Stephen Thomas and George Banting",

year = "1994",

month = nov,

doi = "10.1038/ng1194-285",

language = "English (US)",

volume = "8",

pages = "285--290",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature Research",

number = "3",

}

TY - JOUR

T1 - PBDX is the XG blood group gene

AU - Ellis, Nathan A.

AU - Tippett, Patricia

AU - Petty, Alison

AU - Reid, Marion

AU - Weller, Polly A.

AU - Ye, Tian Z.

AU - German, James

AU - Goodfellow, Peter N.

AU - Thomas, Stephen

AU - Banting, George

PY - 1994/11

Y1 - 1994/11

N2 - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

AB - We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a−) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.

UR - http://www.scopus.com/inward/record.url?scp=0027996829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027996829&partnerID=8YFLogxK

U2 - 10.1038/ng1194-285

DO - 10.1038/ng1194-285

M3 - Article

C2 - 7533029

AN - SCOPUS:0027996829

SN - 1061-4036

VL - 8

SP - 285

EP - 290

JO - Nature Genetics

JF - Nature Genetics

IS - 3

ER -

PBDX is the XG blood group gene

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this