Parameters Affecting High Performance Liquid Chromatographic Separations of Neurohypophyseal Peptide Hormones

Experimental conditions and parameters involved in high performance liquid chromatographic separations of seven neurohypophyseal hormones on several reverse phase columns were investigated. These peptides include arginlne vasotocin, lysine vasopressin, arginine vasopressin, mesotocln, isotocin, oxytocin, and glumitocin. The effects of carbon chain length of the reverse phase support and organic solvent were examined. Using the appropriate solvent system and column, all of the peptides were separated from one another. Separation of the peptides was only part of the goal. The beginning of a study to understand the Interaction of the peptides with the stationary phase as a function of structure was also undertaken. This study led to the conclusion that the major parameters which allow effective separation of these structurally and conformationally closely related peptides are both the eluting strength of the mobile phase and the chemical composition of the stationary phase.