Abstract
Ex vivo differentiation of human hematopoietic stem cells is a widely used model for studying hematopoiesis. The protocol described here is for cytokine induced differentiation of CD34+ hematopoietic stem and progenitor cells to the four myeloid lineage cells. CD34+ cells are isolated from human umbilical cord blood and co-cultured with MS-5 stromal cells in the presence of cytokines. Immunophenotypic characterization of the stem and progenitor cells, and the differentiated myeloid lineage cells are described. Using this protocol, CD34+ cells may be incubated with small molecules or transduced with lentiviruses to express myeloid disease mutations to investigate their impact on myeloid differentiation.
| Original language | English (US) |
|---|---|
| Article number | e59836 |
| Journal | Journal of Visualized Experiments |
| Volume | 2019 |
| Issue number | 150 |
| DOIs | |
| State | Published - Aug 2019 |
Keywords
- CD34 cells
- Common myeloid progenitor
- Developmental Biology
- Erythrocyte
- Flow cytometry
- Granulocyte
- Granulocyte monocyte progenitor
- Hematopoietic stem and progenitor cells
- Issue 150
- Megakaryocyte
- Megakaryocyte erythroid progenitor
- Monocyte
- Myeloid differentiation
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology