Overexpression of ZBP-89, a zinc finger DNA binding protein, in gastric cancer

Toshifumi Taniuchi, Eric R. Mortensen, Amy Ferguson, Joel Greenson, Juanita L. Merchant

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

ZBP-89 is a Kruppel-type zinc finger protein that binds to the gastrin EGF response element (gERE). Sp1 binds to the same DNA element and transactivates gastrin promoter activity, whereas ZBP-89 competes for Sp1 binding and prevents EGF induction. Both transcription factors mediate growth factor signals originating from the EGF receptor and thus were studied in normal and neoplastic tissues or cell lines. When compared to normal tissue from the same patient, ZBP-89 protein expression was increased in neoplastic tissue from the stomach antrum and in malignant cell lines, RT-PCR analysis of ZBP-89 mRNA correlated with protein overexpression. Immunocytochemical studies confirmed that ZBP-89 expression is elevated in neoplastic tissue and chronic gastritis, whereas Sp1 expression was nearly unchanged. These results suggest that the transcription factor ZBP-89, like Sp1, may be a marker for neoplastic transformation in some gastric cancers.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number1
DOIs
StatePublished - Apr 7 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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