TY - JOUR
T1 - Overexpression of ZBP-89, a zinc finger DNA binding protein, in gastric cancer
AU - Taniuchi, Toshifumi
AU - Mortensen, Eric R.
AU - Ferguson, Amy
AU - Greenson, Joel
AU - Merchant, Juanita L.
N1 - Funding Information:
J.L.M. is an investigator of the Howard Hughes Medical Institute. E.R.M. is the recipient of a Robert Wood Johnson Minority Faculty Development Award. These studies were also supported in part by Public Health Service grants to the General Clinical Research Center (M01-RR-00042) and by the Tissue Procurement Core of the University of Michigan Comprehensive Cancer Center (CA46952). Oligonucleotides were synthesized by the University of Michigan DNA synthesis core facility. The authors acknowledge the assistance of Gail Kelsey in preparing the manuscript.
PY - 1997/4/7
Y1 - 1997/4/7
N2 - ZBP-89 is a Kruppel-type zinc finger protein that binds to the gastrin EGF response element (gERE). Sp1 binds to the same DNA element and transactivates gastrin promoter activity, whereas ZBP-89 competes for Sp1 binding and prevents EGF induction. Both transcription factors mediate growth factor signals originating from the EGF receptor and thus were studied in normal and neoplastic tissues or cell lines. When compared to normal tissue from the same patient, ZBP-89 protein expression was increased in neoplastic tissue from the stomach antrum and in malignant cell lines, RT-PCR analysis of ZBP-89 mRNA correlated with protein overexpression. Immunocytochemical studies confirmed that ZBP-89 expression is elevated in neoplastic tissue and chronic gastritis, whereas Sp1 expression was nearly unchanged. These results suggest that the transcription factor ZBP-89, like Sp1, may be a marker for neoplastic transformation in some gastric cancers.
AB - ZBP-89 is a Kruppel-type zinc finger protein that binds to the gastrin EGF response element (gERE). Sp1 binds to the same DNA element and transactivates gastrin promoter activity, whereas ZBP-89 competes for Sp1 binding and prevents EGF induction. Both transcription factors mediate growth factor signals originating from the EGF receptor and thus were studied in normal and neoplastic tissues or cell lines. When compared to normal tissue from the same patient, ZBP-89 protein expression was increased in neoplastic tissue from the stomach antrum and in malignant cell lines, RT-PCR analysis of ZBP-89 mRNA correlated with protein overexpression. Immunocytochemical studies confirmed that ZBP-89 expression is elevated in neoplastic tissue and chronic gastritis, whereas Sp1 expression was nearly unchanged. These results suggest that the transcription factor ZBP-89, like Sp1, may be a marker for neoplastic transformation in some gastric cancers.
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U2 - 10.1006/bbrc.1997.6310
DO - 10.1006/bbrc.1997.6310
M3 - Article
C2 - 9144414
AN - SCOPUS:0031557691
SN - 0006-291X
VL - 233
SP - 154
EP - 160
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -