Osteomyocutaneous flap as a preclinical composite tissue allograft: Swine model

Ren Xiaoping, Mansour V. Shirbacheh, E. Tuncay Üstüner, Marty Zdichavsky, Jean Edelstein, Claudio Maldonado, Warren C. Breidenbach, Johannes Frank, Gordon R. Tobin, Jon W. Jones, John H. Barker

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Composite tissue allotransplantation (CTA) constitutes one of the last frontiers of microsurgery. Prior to its clinical application, the long-term efficacy of modern immunotherapy must be tested in a pre-clinical CTA model. Based on the concept of osteomyocutaneous forearm flap, we developed a CTA flap model in swine. After identifying the vascular territory of the flaps in six pigs (vascular casting), flaps were transplanted from mismatched donor to recipient pigs (n = 6). Rejection was assessed daily by visual inspection and histopathology of biopsy specimens. Recipient pigs were able to ambulate immediately following surgery. There were no flap failures owing to technical or surgical complications. Rejection occurred over a period of 7 days as manifested by edema, cellular infiltration, epidermalysis, and thrombosis. This pre-clinical flap model is excellent for evaluating the effectiveness of modern immunotherapy because it is anatomically and immunologically relevant and because the minimal morbidity caused to the animal permits long-term studies. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)143-149
Number of pages7
Issue number3
StatePublished - 2000

ASJC Scopus subject areas

  • Surgery


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