Orthotopic aortic transplantation: A rat model to study the development of chronic vasculopathy

Mandy Stubbendorff, Tobias Deuse, Anna Hammel, Robert C. Robbins, Hermann Reichenspurner, Sonja Schrepfer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey (3) published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model (1, 2). In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated (2). The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting (4). A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models (5).

Original languageEnglish (US)
Article numbere1989
JournalJournal of Visualized Experiments
Issue number46
DOIs
StatePublished - Dec 2010
Externally publishedYes

Keywords

  • Chronic rejection
  • Issue 46
  • Medicine
  • Rat
  • Transplant vasculopathy
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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