Optomechanical measurement of the role of lamins in whole cell deformability

Thorsten Kolb, Julia Kraxner, Kai Skodzek, Michael Haug, Dean Crawford, Kendra K. Maaß, Katerina E. Aifantis, Graeme Whyte

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell.

Original languageEnglish (US)
Pages (from-to)1657-1664
Number of pages8
JournalJournal of Biophotonics
Issue number12
StatePublished - Dec 2017


  • Cell Mechanics
  • Lamin A
  • Nuclear Envelope
  • Optical Stretcher
  • Single-Cell Analysis

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry, Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy


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