Abstract
Optimum growth conditions and pH control solution were obtained in the biosynthesis of PHB(poly-β-hydroxybutyrate) with Alcaligenes eutrophus. Optimum carbon and nitrogen sources were fructose and (NH4) 2SO4, respectively, and optimum additive was 1:1 mixture of yeast extract and polypeptone. To select the optimum pH control solution, various pH control solutions (NaOH, KOH, Na2CO3, and NaOH+KOH) were tested. Na2CO3 solution was found to be the best out of the four solutions tested, because CO2 generated from Na2CO3 could be used as a carbon source. Mixture of NaOH and KOH showed better results than the solution of NaOH or KOH alone. This could be due to the balanced amount of cations (Na+ and K+), which might promote not only the permeation of substrates, but also pumping out Na+ ions.
Original language | English (US) |
---|---|
Pages (from-to) | 215-220 |
Number of pages | 6 |
Journal | Journal of Industrial and Engineering Chemistry |
Volume | 4 |
Issue number | 3 |
State | Published - 1998 |
Externally published | Yes |
Keywords
- A. eutrophus
- PHB
- pH control solution
ASJC Scopus subject areas
- General Chemical Engineering