Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

Samantha M. Hill; Marco Padilla-Rodriguez; Amber Clements; Jillian A. Sweetland; Sara S. Parker; Noel A. Warfel; Ghassan Mouneimne

doi:10.1016/j.xpro.2022.101516

Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

Samantha M. Hill, Marco Padilla-Rodriguez, Amber Clements, Jillian A. Sweetland, Sara S. Parker, Noel A. Warfel, Ghassan Mouneimne

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).

Original language	English (US)
Article number	101516
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101516
State	Published - Sep 16 2022

Keywords

Biophysics
Cancer
Cell Biology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.101516

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title = "Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior",

abstract = "We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).",

keywords = "Biophysics, Cancer, Cell Biology",

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doi = "10.1016/j.xpro.2022.101516",

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T1 - Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

AU - Hill, Samantha M.

AU - Padilla-Rodriguez, Marco

AU - Clements, Amber

AU - Sweetland, Jillian A.

AU - Parker, Sara S.

AU - Warfel, Noel A.

AU - Mouneimne, Ghassan

PY - 2022/9/16

Y1 - 2022/9/16

N2 - We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).

AB - We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).

KW - Biophysics

KW - Cancer

KW - Cell Biology

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Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

Abstract

Keywords

ASJC Scopus subject areas

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