Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior

Samantha M. Hill, Marco Padilla-Rodriguez, Amber Clements, Jillian A. Sweetland, Sara S. Parker, Noel A. Warfel, Ghassan Mouneimne

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).

Original languageEnglish (US)
Article number101516
JournalSTAR Protocols
Volume3
Issue number3
DOIs
StatePublished - Sep 16 2022

Keywords

  • Biophysics
  • Cancer
  • Cell Biology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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