Optimization of manganese peroxidase production by the white rot fungus Bjerkandera sp. strain BOS55

Tünde Mester, Jim A. Field

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Manganese dependent peroxidase (MnP) is the most ubiquitous peroxidase produced by white rot fungi. MnP is known to be involved in lignin degradation, biobleaching and in the oxidation of hazardous organopollutants. Bjerkandera sp. strain BOS55 is a nitrogen-unregulated white rot fungus which produces high amounts of MnP in the excess of N-nutrients due to increased biomass yield. Therefore, the strain is a good candidate for use in large scale production of this enzyme. The objective of this study was to optimize the MnP production in N-sufficient cultures by varying different physiological factors such as Mn concentration, culture pH, incubation temperature and the addition of organic acids. The fungus produced the highest level of MnP (up to 900 U 1-1) when the Mn concentration was 0.2 to 1 mM, the pH value was 5.2, and the incubation temperature was 30°C. A noteworthy finding was that MnP was also produced at lower levels in the complete absence of Mn. The addition of organic acids like glycolate, malonate, glucuronate, gluconate, 2-hydroxybutyrate to the culture medium increased the peak titres of MnP up to 1250 U 1-1. FPLC profiles indicated that the organic acids stimulated the production of all MnP isoenzymes present in the extracellular fluid of the fungus.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Oct 15 1997


  • Lignin degradation
  • Manganese peroxidase
  • White rot fungi

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


Dive into the research topics of 'Optimization of manganese peroxidase production by the white rot fungus Bjerkandera sp. strain BOS55'. Together they form a unique fingerprint.

Cite this