TY - JOUR
T1 - Optimization of an Antibody Microarray Printing Process Using a Designed Experiment
AU - Summers, Alexander J.
AU - Devadhasan, Jasmine P.
AU - Gu, Jian
AU - Montgomery, Douglas C.
AU - Fischer, Brittany
AU - Gates-Hollingsworth, Marcellene A.
AU - Pflughoeft, Kathryn J.
AU - Vo-Dinh, Tuan
AU - Aucoin, David P.
AU - Zenhausern, Frederic
N1 - Funding Information:
Special thank you to Sujata Pandit, Derrick Hau, Ali Fattahi, and Supriya Atta for their expertize and contribution to our study and assistance in writing the manuscript. This work was supported by the Chemical Biological Technologies Directorate [Contract # HDTRA1-16-C-0026] and the Advanced Technology International [Contract # MCDC-18-04-09-002] from the Department of Defense Chemical and Biological Defense program through the Defense Threat Reduction Agency (DTRA).
Funding Information:
This work was supported by the Chemical Biological Technologies Directorate [Contract # HDTRA1-16-C-0026] and the Advanced Technology International [Contract # MCDC-18-04-09-002] from the Department of Defense Chemical and Biological Defense program through the Defense Threat Reduction Agency (DTRA).
Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.
PY - 2022
Y1 - 2022
N2 - Antibody microarrays have proven useful in immunoassay-based point-of-care diagnostics for infectious diseases. Noncontact piezoelectric inkjet printing has advantages to print antibody microarrays on nitrocellulose substrates for this application due to its compatibility with sensitive solutions and substrates, simple droplet control, and potential for high-capacity printing. However, there remain real-world challenges in printing such microarrays, which motivated this study. The effects of three concentrations of capture antibody (cAb) reagents and nozzle hydrostatic pressures were chosen to investigate three responses: the number of printed membrane disks, dispensing performance, and microarray quality. Printing conditions were found to be most ideal with 5 mg/mL cAb and a nozzle hydrostatic pressure near zero, which produced 130 membrane disks in a single print versus the 10 membrane disks per print before optimization. These results serve to inform efficient printing of antibody microarrays on nitrocellulose membranes for rapid immunoassay-based detection of infectious diseases and beyond.
AB - Antibody microarrays have proven useful in immunoassay-based point-of-care diagnostics for infectious diseases. Noncontact piezoelectric inkjet printing has advantages to print antibody microarrays on nitrocellulose substrates for this application due to its compatibility with sensitive solutions and substrates, simple droplet control, and potential for high-capacity printing. However, there remain real-world challenges in printing such microarrays, which motivated this study. The effects of three concentrations of capture antibody (cAb) reagents and nozzle hydrostatic pressures were chosen to investigate three responses: the number of printed membrane disks, dispensing performance, and microarray quality. Printing conditions were found to be most ideal with 5 mg/mL cAb and a nozzle hydrostatic pressure near zero, which produced 130 membrane disks in a single print versus the 10 membrane disks per print before optimization. These results serve to inform efficient printing of antibody microarrays on nitrocellulose membranes for rapid immunoassay-based detection of infectious diseases and beyond.
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U2 - 10.1021/acsomega.2c03595
DO - 10.1021/acsomega.2c03595
M3 - Article
AN - SCOPUS:85137697576
JO - ACS Omega
JF - ACS Omega
SN - 2470-1343
ER -