Optical detection of molecular beacons in microfluidic devices

Michal Balberg, Rrassimira Hristova, David J. Brady, David J. Beebe, L. Raskin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Hybridization and detection of E. coli ribosomal RNA with molecular beacons labeled with a fluorescent dye Is achieved in a microfluidic device. The device consists of a four ports mixing chamber, where the probes and the target molecules mix by diffusion. The weak fluorescent signal is detected by two large core optical fibers that are placed on both sides of the channel and collect the light emitted in both directions. The fibers are coupled to a spectrophotometer. The detection limit for the system is 0.2 ftnol.

Original languageEnglish (US)
Title of host publication1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsAndre Dittmar, David Beebe
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages425-428
Number of pages4
ISBN (Electronic)0780366034, 9780780366039
DOIs
StatePublished - 2000
Externally publishedYes
Event1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000 - Lyon, France
Duration: Oct 12 2000Oct 14 2000

Publication series

Name1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000
Country/TerritoryFrance
CityLyon
Period10/12/0010/14/00

ASJC Scopus subject areas

  • Engineering(all)

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