One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun; Carolyn A. Haller; Xiao Yi Wu; Vincent P. Conticello; Elliot L. Chaikof

doi:10.1021/pr050245g

One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Xue Long Sun, Carolyn A. Haller, Xiao Yi Wu, Vincent P. Conticello, Elliot L. Chaikof

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and ¹H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

Original language	English (US)
Pages (from-to)	2355-2359
Number of pages	5
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr050245g
State	Published - Nov 2005
Externally published	Yes

Keywords

Elastin-like polypeptide
Glyco-affinity precipitation
Glycoconjugate
Protein purification

ASJC Scopus subject areas

General Chemistry
Biochemistry

Access to Document

10.1021/pr050245g

Cite this

@article{11e35c978afe409ebcb957857487d42a,

title = "One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation",

abstract = "A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.",

keywords = "Elastin-like polypeptide, Glyco-affinity precipitation, Glycoconjugate, Protein purification",

author = "Sun, {Xue Long} and Haller, {Carolyn A.} and Wu, {Xiao Yi} and Conticello, {Vincent P.} and Chaikof, {Elliot L.}",

year = "2005",

month = nov,

doi = "10.1021/pr050245g",

language = "English (US)",

volume = "4",

pages = "2355--2359",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - One-pot glyco-affinity precipitation purification for enhanced proteomics

T2 - The flexible alignment of solution-phase capture/release and solid-phase separation

AU - Sun, Xue Long

AU - Haller, Carolyn A.

AU - Wu, Xiao Yi

AU - Conticello, Vincent P.

AU - Chaikof, Elliot L.

PY - 2005/11

Y1 - 2005/11

N2 - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

AB - A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and 1H NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.

KW - Elastin-like polypeptide

KW - Glyco-affinity precipitation

KW - Glycoconjugate

KW - Protein purification

UR - http://www.scopus.com/inward/record.url?scp=29144478731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144478731&partnerID=8YFLogxK

U2 - 10.1021/pr050245g

DO - 10.1021/pr050245g

M3 - Article

C2 - 16335985

AN - SCOPUS:29144478731

SN - 1535-3893

VL - 4

SP - 2355

EP - 2359

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 6

ER -

One-pot glyco-affinity precipitation purification for enhanced proteomics: The flexible alignment of solution-phase capture/release and solid-phase separation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this