Oncogenic transformation of human-derived gastric organoids

Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, Yana Zavros

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages205-213
Number of pages9
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1576
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CRISPR/Cas-9
  • Human fundic gastric organoids
  • Nucleofection
  • Organoid orthotopic transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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