On the Quantification of Cellular Velocity Fields

Dhruv K. Vig, Alex E. Hamby, Charles W. Wolgemuth

Research output: Contribution to journalReview articlepeer-review

39 Scopus citations


The application of flow visualization in biological systems is becoming increasingly common in studies ranging from intracellular transport to the movements of whole organisms. In cell biology, the standard method for measuring cell-scale flows and/or displacements has been particle image velocimetry (PIV); however, alternative methods exist, such as optical flow constraint. Here we review PIV and optical flow, focusing on the accuracy and efficiency of these methods in the context of cellular biophysics. Although optical flow is not as common, a relatively simple implementation of this method can outperform PIV and is easily augmented to extract additional biophysical/chemical information such as local vorticity or net polymerization rates from speckle microscopy.

Original languageEnglish (US)
Pages (from-to)1469-1475
Number of pages7
JournalBiophysical Journal
Issue number7
StatePublished - Apr 12 2016

ASJC Scopus subject areas

  • Biophysics


Dive into the research topics of 'On the Quantification of Cellular Velocity Fields'. Together they form a unique fingerprint.

Cite this