TY - GEN
T1 - On the minimum detection limit of circulating tumor cells in an antibody-functionalized microchannel array
AU - Zheng, Xiangjun
AU - Jiang, Linan
AU - Schroeder, Joyce A.
AU - Marron, Marilyn
AU - Iannone, Maria E
AU - Stopeck, Alison T
AU - Zohar, Yitshak
PY - 2012
Y1 - 2012
N2 - The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1 to 1:100,000. The dependence of the device performance, particularly its sensitivity and specificity in isolating target cells, on the applied flow shear rate is characterized. The results demonstrate capture of circulating tumor cells from mixtures with high sensitivity and high specificity. The multichannel array devices allow testing of samples within a shorter time, which is critical for high throughput applications.
AB - The minimum detection limit in isolation of circulating tumor cells (CTCs) from binary mixtures is experimentally explored utilizing microchannel arrays functionalized with EpCAM antibodies. Hence, the tested binary mixtures contained EpCAM-positive and EpCAM-negative cells serving as target and non-target cells, respectively. The ratio of target to non-target cells in the mixtures ranged from 1:1 to 1:100,000. The dependence of the device performance, particularly its sensitivity and specificity in isolating target cells, on the applied flow shear rate is characterized. The results demonstrate capture of circulating tumor cells from mixtures with high sensitivity and high specificity. The multichannel array devices allow testing of samples within a shorter time, which is critical for high throughput applications.
KW - Bio-functional microchannel array
KW - Circulating tumor cells
KW - High throughput
KW - Minimum detection limit
UR - http://www.scopus.com/inward/record.url?scp=84880197136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880197136&partnerID=8YFLogxK
U2 - 10.1109/NANOMED.2012.6509115
DO - 10.1109/NANOMED.2012.6509115
M3 - Conference contribution
AN - SCOPUS:84880197136
SN - 9781467351027
T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
SP - 87
EP - 91
BT - NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
T2 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
Y2 - 4 November 2012 through 7 November 2012
ER -