Ocean viruses: Rigorously evaluating the metagenomic sample-to-sequence pipeline

Melissa B. Duhaime, Matthew B. Sullivan

Research output: Contribution to journalReview articlepeer-review

66 Scopus citations


As new environments are studied, viruses consistently emerge as important and prominent players in natural and man-made ecosystems. However, much of what we know is built both upon the foundation of the culturable minority and using methods that are often insufficiently ground-truthed. Here, we review the modern culture-independent viral metagenomic sample-to-sequence pipeline and how next-generation sequencing techniques are drastically altering our ability to systematically and rigorously evaluate them. Together, a series of studies quantitatively evaluate existing and new methods that allow-even for ultra-low DNA samples-the generation of replicable, near-quantitative datasets that maximize inter-comparability and biological inference.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
Issue number2
StatePublished - Dec 20 2012


  • Environmental virology
  • Metagenomics
  • Next-generation sequencing
  • Ocean viruses
  • Viral concentration
  • Viral ecology
  • Viral purification

ASJC Scopus subject areas

  • Virology


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