Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Masaaki Kitajima; Andri T. Rachmadi; Brandon C. Iker; Eiji Haramoto; Ian L. Pepper; Charles P. Gerba

doi:10.1007/s00705-015-2435-x

Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Masaaki Kitajima
, Andri T. Rachmadi
, Brandon C. Iker
, Eiji Haramoto
, Ian L. Pepper
, Charles P. Gerba

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 10⁵ and 1.54 × 10³ copies/liter in influent and effluent, respectively. The strains were characterized based on their 5’ untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.

Original language	English (US)
Pages (from-to)	1775-1779
Number of pages	5
Journal	Archives of virology
Volume	160
Issue number	7
DOIs	https://doi.org/10.1007/s00705-015-2435-x
State	Published - Jul 15 2015

Keywords

Cosavirus
Nested PCR
Phylogenetic analysis
Wastewater
qPCR

ASJC Scopus subject areas

Virology

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10.1007/s00705-015-2435-x

Cite this

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title = "Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States",

abstract = "Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 \%) influent samples and one (4 \%) effluent sample, with the highest concentration of 3.24 × 105 and 1.54 × 103 copies/liter in influent and effluent, respectively. The strains were characterized based on their 5{\textquoteright} untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.",

keywords = "Cosavirus, Nested PCR, Phylogenetic analysis, Wastewater, qPCR",

author = "Masaaki Kitajima and Rachmadi, \{Andri T.\} and Iker, \{Brandon C.\} and Eiji Haramoto and Pepper, \{Ian L.\} and Gerba, \{Charles P.\}",

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T1 - Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

AU - Kitajima, Masaaki

AU - Rachmadi, Andri T.

AU - Iker, Brandon C.

AU - Haramoto, Eiji

AU - Pepper, Ian L.

AU - Gerba, Charles P.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 105 and 1.54 × 103 copies/liter in influent and effluent, respectively. The strains were characterized based on their 5’ untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.

AB - Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 105 and 1.54 × 103 copies/liter in influent and effluent, respectively. The strains were characterized based on their 5’ untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.

KW - Cosavirus

KW - Nested PCR

KW - Phylogenetic analysis

KW - Wastewater

KW - qPCR

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Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Abstract

Keywords

ASJC Scopus subject areas

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