Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Masaaki Kitajima; Andri T. Rachmadi; Brandon C. Iker; Eiji Haramoto; Ian L. Pepper; Charles P. Gerba

doi:10.1007/s00705-015-2435-x

Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

Masaaki Kitajima, Andri T. Rachmadi, Brandon C. Iker, Eiji Haramoto, Ian L. Pepper, Charles P. Gerba

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 10⁵ and 1.54 × 10³ copies/liter in influent and effluent, respectively. The strains were characterized based on their 5’ untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.

Original language	English (US)
Pages (from-to)	1775-1779
Number of pages	5
Journal	Archives of virology
Volume	160
Issue number	7
DOIs	https://doi.org/10.1007/s00705-015-2435-x
State	Published - Jul 15 2015

Keywords

Cosavirus
Nested PCR
Phylogenetic analysis
Wastewater
qPCR

ASJC Scopus subject areas

Virology

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10.1007/s00705-015-2435-x

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Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States. / Kitajima, Masaaki; Rachmadi, Andri T.; Iker, Brandon C.; Haramoto, Eiji; Pepper, Ian L.; Gerba, Charles P.

In: Archives of virology, Vol. 160, No. 7, 15.07.2015, p. 1775-1779.

Research output: Contribution to journal › Article › peer-review

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abstract = "Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 × 105 and 1.54 × 103 copies/liter in influent and effluent, respectively. The strains were characterized based on their 5{\textquoteright} untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.",

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author = "Masaaki Kitajima and Rachmadi, {Andri T.} and Iker, {Brandon C.} and Eiji Haramoto and Pepper, {Ian L.} and Gerba, {Charles P.}",

note = "Funding Information: This study was partly supported by the National Science Foundation (NSF) Water and Environmental Technology (WET) Center, The University of Arizona. Funding Information: We also wish to acknowledge the support of the Japan Society for the Promotion of Science (JSPS) to Masaaki Kitajima, under JSPS Postdoctoral Fellowships for Research Abroad (FY 2011 no. 517). Andri T. Rachmadi is a recipient of a 2012 Fulbright Master of Science and Technology Scholarship. Publisher Copyright: {\textcopyright} 2015, Springer-Verlag Wien.",

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AU - Haramoto, Eiji

AU - Pepper, Ian L.

AU - Gerba, Charles P.

N1 - Funding Information: This study was partly supported by the National Science Foundation (NSF) Water and Environmental Technology (WET) Center, The University of Arizona. Funding Information: We also wish to acknowledge the support of the Japan Society for the Promotion of Science (JSPS) to Masaaki Kitajima, under JSPS Postdoctoral Fellowships for Research Abroad (FY 2011 no. 517). Andri T. Rachmadi is a recipient of a 2012 Fulbright Master of Science and Technology Scholarship. Publisher Copyright: © 2015, Springer-Verlag Wien.

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Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States

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