Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a numberof variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FFT intensity to the intensity of an orthogonal angle, giving a numerical value of the alignment of the microscopic structures. This allows for a more objective analysis than alternative approaches, which rely upon relative intensities.
|Original language||English (US)|
|Journal||Current Protocols in Essential Laboratory Techniques|
|State||Published - 2013|
- Fast fourier transformation
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)