Abstract
Quantification of immunohistochemistry (IHC) and immunofluorescence (IF) using image intensity depends on a numberof variables. These variables add a subjective complexity in keeping a standard within and between laboratories. Fast Fourier Transformation (FFT) algorithms, however, allow for a rapid and objective quantification (via statistical analysis) using cell morphologies when the microscopic structures are oriented or aligned. Quantification of alignment is given in terms of a ratio of FFT intensity to the intensity of an orthogonal angle, giving a numerical value of the alignment of the microscopic structures. This allows for a more objective analysis than alternative approaches, which rely upon relative intensities.
Original language | English (US) |
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Article number | 9.5 |
Journal | Current Protocols in Essential Laboratory Techniques |
Volume | 2013 |
Issue number | SUPPL.7 |
DOIs | |
State | Published - 2013 |
Keywords
- Fast fourier transformation
- Immunohistochemistry
- Microscopy
- Quantification
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)