TY - JOUR
T1 - Numerical simulation of long-range cell manipulation with AC electrokinetics by immersed boundary-lattice Boltzmann method
AU - Ren, Qinlong
AU - Meng, Fanlong
AU - Chan, Cho Lik
N1 - Funding Information:
The work was supported by the China Postdoctoral Science Foundation Funded Project (No. 2017M623169).
Publisher Copyright:
© 2018 International Heat Transfer Conference. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Alternating current (AC) electrokinetics have been widely used to manipulate bioparticles such as mammalian cell, bacteria, DNA, and protein among which the efficient cell patterning is essential for several biomedical and tissue engineering applications. Dielectrophoresis (DEP) is a well-known phenomenon that a force acts on the polarized particle in the non-uniform electric field, and it has been demonstrated as an effective, noninvasive, and non-destructive technique for cell trapping and separation. However, DEP force reduces dramatically with the decrease of electric field gradients which makes it ineffective for controlling the motion of cell in the region far away from the electrode edges. Fortunately, AC electrothermal (ACET) flow occurs when there exists electric field and temperature gradient caused by Joule heating or other external heat sources. The driving mechanism of ACET flow makes it important for pumping the physiological media of high conductivity under which circumstance the AC electroosmosis flow is weak. The drag force induced by ACET flow on the cell could transport it into the region near the electrode where the negative DEP force is significant. By balancing the DEP force, the drag force, and the gravitational force, the cell could be successfully suspended in the solution which is useful for cell patterning or other biomedical operations. In the current work, the immersed boundary-lattice Boltzmann method (IB-LBM) with GPU acceleration on a CUDA computational platform is used to accurately simulate the motion of cell under hybrid AC electrokinetic phenomena. The results indicate that a single cell could be successfully suspended at an equilibrium position due to the balanced ACET electrohydrodynamic force, negative DEP force, and gravitational force. However, the double cells confront a periodic motion around each other when the DEP force is not sufficient to overcome the effects of ACET flow and cell-cell interaction.
AB - Alternating current (AC) electrokinetics have been widely used to manipulate bioparticles such as mammalian cell, bacteria, DNA, and protein among which the efficient cell patterning is essential for several biomedical and tissue engineering applications. Dielectrophoresis (DEP) is a well-known phenomenon that a force acts on the polarized particle in the non-uniform electric field, and it has been demonstrated as an effective, noninvasive, and non-destructive technique for cell trapping and separation. However, DEP force reduces dramatically with the decrease of electric field gradients which makes it ineffective for controlling the motion of cell in the region far away from the electrode edges. Fortunately, AC electrothermal (ACET) flow occurs when there exists electric field and temperature gradient caused by Joule heating or other external heat sources. The driving mechanism of ACET flow makes it important for pumping the physiological media of high conductivity under which circumstance the AC electroosmosis flow is weak. The drag force induced by ACET flow on the cell could transport it into the region near the electrode where the negative DEP force is significant. By balancing the DEP force, the drag force, and the gravitational force, the cell could be successfully suspended in the solution which is useful for cell patterning or other biomedical operations. In the current work, the immersed boundary-lattice Boltzmann method (IB-LBM) with GPU acceleration on a CUDA computational platform is used to accurately simulate the motion of cell under hybrid AC electrokinetic phenomena. The results indicate that a single cell could be successfully suspended at an equilibrium position due to the balanced ACET electrohydrodynamic force, negative DEP force, and gravitational force. However, the double cells confront a periodic motion around each other when the DEP force is not sufficient to overcome the effects of ACET flow and cell-cell interaction.
KW - AC electrothermal flow
KW - Cell manipulation
KW - Dielectrophoresis
KW - Immersed boundary method
KW - Lattice Boltzmann method
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U2 - 10.1615/ihtc16.bma.022742
DO - 10.1615/ihtc16.bma.022742
M3 - Conference article
AN - SCOPUS:85068328042
SN - 2377-424X
VL - 2018-August
SP - 531
EP - 539
JO - International Heat Transfer Conference
JF - International Heat Transfer Conference
T2 - 16th International Heat Transfer Conference, IHTC 2018
Y2 - 10 August 2018 through 15 August 2018
ER -