Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA

Carol L. Dieckmann; Telsa M. Mittelmeier

doi:10.1007/BF00434815

Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA

Carol L. Dieckmann, Telsa M. Mittelmeier

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27 Scopus citations

Abstract

CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.

Original language	English (US)
Pages (from-to)	391-397
Number of pages	7
Journal	Current Genetics
Volume	12
Issue number	6
DOIs	https://doi.org/10.1007/BF00434815
State	Published - Oct 1987

Keywords

Mitochondrial gene fusions
Organelle biogenesis
RNA processing
Yeast mitochondria

ASJC Scopus subject areas

Genetics

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10.1007/BF00434815

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title = "Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA",

abstract = "CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.",

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T1 - Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA

AU - Dieckmann, Carol L.

AU - Mittelmeier, Telsa M.

PY - 1987/10

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N2 - CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.

AB - CBP1 is a nuclearly-encoded protein that is imported into mitochondria and confers stability on the mRNA for cytochrome b. Previous work has shown that CBP1 interacts with the cytochrome b transcript upstream of the coding sequence; a region encompassing some 1,100 nucleotides. The work presented here narrows the site of action of CBP1 to the distal third of this upstream sequence through analysis of mRNA produced from a novel recombinant gene containing segments of the gene for cytochrome b, cob, and the ATP synthase subunit 9 gene, olil. In a wild-type CBP1 strain, the cob-olil-cob gene produces stable, mature mRNA that is translated and contributes a portion of the cytochrome b necessary for optimal growth on non-fermentable medium.

KW - Mitochondrial gene fusions

KW - Organelle biogenesis

KW - RNA processing

KW - Yeast mitochondria

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Nuclearly-encoded CBP1 interacts with the 5′ end of mitochondrial cytochrome b pre-mRNA

Abstract

Keywords

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