Nuclear microsatellite loci for Arabidopsis halleri (Brassicaceae), a model species to study plant adaptation to heavy metals

Cécile Godé, Isabelle Decombeix, Alicja Kostecka, Pawel Wasowicz, Maxime Pauwels, Adeline Courseaux, Pierre Saumitou-Laprade

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Premise of the study: Arabidopsis halleri is a model species to study the adaptation of plants to soils contaminated by zinc, cadmium, and lead. To provide a neutral genetic background with which adaptive genetic markers could be compared, we developed highly polymorphic neutral microsatellite markers. Methods and Results: Using a microsatellite-enriched library method, we identified 120 microsatellite loci for quantitative trait locus (QTL) mapping analysis, of which eight primer pairs were developed in a single multiplex for population genetic studies. Analyses were performed on 508 individuals from 26 populations. All loci were polymorphic with six to 23 alleles per locus. Genetic diversity varied between 0.56 and 0.76. Conclusions: Our results demonstrated the value of these eight microsatellite markers to investigate neutral population genetic structure in A. halleri. To increase the resolution of population genetic analyses, we suggest adding them to the 11 markers previously developed independently.

Original languageEnglish (US)
Pages (from-to)e49-e52
JournalAmerican journal of botany
Volume99
Issue number2
DOIs
StatePublished - Feb 2012
Externally publishedYes

Keywords

  • Arabidopsis halleri
  • Brassicaceae
  • Demographic processes
  • Microsatellite markers
  • Population genetic structure
  • Pseudometallophyte

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

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