TY - JOUR
T1 - Nuclear and cytoplasmic receptors for 1,25-dihydroxycholecalciferol in intestinal mucosa
AU - Brumbaugh, Peter F.
AU - Haussler, Mark R.
N1 - Funding Information:
fraction of intestinal mucosa. Further investigation of the chromatin-+Supported by USPHSG rant AM 15781, USPHST raining Grant GM 01982 and General Research Support Grant RR 05675 of the University of Arizona, College of Medicine. *Abbreviations used: 25-OH-CC, 25-hydroxycholecalciferol; 1,25-(OH)2-CC, 1,25-dihydroxycholecalciferol; sucrose-Tris-K-Mg, 0.25 M Sucrose in 0.05 M Tris-HCl, pH 7.4, 0.025 M KCl, 0.005 M Mgc12.
PY - 1973/3/5
Y1 - 1973/3/5
N2 - The apparent hormonal form of cholecalciferol, 1,25-dihydroxycholecalciferol (1,25-(OH)2-CC), was incubated with intestinal mucosa homogenates and whole intestinal tissue, in vitro. After 40-70 min, 1,25-(OH)2-CC was specifically associated with the nuclear chromatin fraction. This sterol remains bound to the cytosol fraction at 0°C and a dramatic movement to the nuclear chromatin occurs at 37°C indicating that the subcellular localization of the sterol is temperature dependent. Isolated intestinal cytosol, previously incubated with 1,25-(OH)2-CC, is required for transportation of the hormone to the intestinal chromatin fraction; cytosol fractions from other tissues are ineffective mediators of this sterol migration. It is concluded that the intestinal cytosol contains a specific receptor that functions to transport 1,25-(OH)2-CC to the nucleus, its probable site of action.
AB - The apparent hormonal form of cholecalciferol, 1,25-dihydroxycholecalciferol (1,25-(OH)2-CC), was incubated with intestinal mucosa homogenates and whole intestinal tissue, in vitro. After 40-70 min, 1,25-(OH)2-CC was specifically associated with the nuclear chromatin fraction. This sterol remains bound to the cytosol fraction at 0°C and a dramatic movement to the nuclear chromatin occurs at 37°C indicating that the subcellular localization of the sterol is temperature dependent. Isolated intestinal cytosol, previously incubated with 1,25-(OH)2-CC, is required for transportation of the hormone to the intestinal chromatin fraction; cytosol fractions from other tissues are ineffective mediators of this sterol migration. It is concluded that the intestinal cytosol contains a specific receptor that functions to transport 1,25-(OH)2-CC to the nucleus, its probable site of action.
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U2 - 10.1016/0006-291X(73)90509-3
DO - 10.1016/0006-291X(73)90509-3
M3 - Article
C2 - 4699566
AN - SCOPUS:0015914645
SN - 0006-291X
VL - 51
SP - 74
EP - 80
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -