@inproceedings{bfa831dfc67e41e38b6f99629c6627e9,
title = "Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion",
abstract = "Nonlinear structured illumination microscopy (SIM) allows full-field imaging at resolutions <100 nm. Two nonlinear effects, excitation saturation (SSIM) and the photo-switching of protein had been applied to nonlinear SIM. We report a new SIM technique which utilizes the nonlinearity of STED effect. Resolution and signal noise ratio simulation shows that STED-SIM may serve as a better alternative to SSIM and SIM with photo-switchable protein. SIM requires a strong nonlinear effect in a large area. We use Surface Plasmon Resonant to enhance of evanescence field near a dielectric-metal-dielectric interface. An 8 times STED effect enhancement is achieved on an optimized glass-silver-glass- water planar structure. We further use the interference of two SPR-enhanced STED fields propagating at opposite direction to generate a 1D structured STED field. Combined with a uniform excitation field, the structure STED field allows full field total internal reflection imaging with an enhanced resolution along the structured dimension. Less than 50 nm resolution is demonstrated. A STED-SIM microscope with 2D structured STED field is under development. Future research will apply the microscope to superresolution imaging of membrane resident or near membrane structure at super-resolution in live cells.",
keywords = "SPR, STED, Structured illumination microscopy, superresolution",
author = "Han Zhang and Ming Zhao and Leilei Peng",
year = "2013",
doi = "10.1117/12.2005043",
language = "English (US)",
isbn = "9780819493590",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Single Molecule Spectroscopy and Superresolution Imaging VI",
note = "Single Molecule Spectroscopy and Superresolution Imaging VI ; Conference date: 02-02-2013 Through 03-02-2013",
}