NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

Clement Lin; Jonathan Dickerhoff; Danzhou Yang

doi:10.1007/978-1-4939-9666-7_9

NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

Clement Lin, Jonathan Dickerhoff, Danzhou Yang

Pharmacology and Toxicology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

33 Scopus citations

Abstract

G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	157-176
Number of pages	20
DOIs	https://doi.org/10.1007/978-1-4939-9666-7_9
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	2035
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

G-quadruplex structures
G-quadruplex-interactive compounds
NMR spectroscopy

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9666-7_9

Cite this

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abstract = "G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.",

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AU - Dickerhoff, Jonathan

AU - Yang, Danzhou

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

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AB - G-quadruplexes are noncanonical, four-stranded nucleic acid secondary structures formed in sequences containing consecutive runs of guanines. These G-quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method for understanding the structures of G-quadruplexes and their interactions with small molecules under physiologically relevant conditions. Here, we present the NMR methodology used in our research group for the study of DNA G-quadruplex structures in physiologically relevant solution and their ligand interactions.

KW - G-quadruplex structures

KW - G-quadruplex-interactive compounds

KW - NMR spectroscopy

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NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

Abstract

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Keywords

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