TY - JOUR
T1 - [Nle4, D-Phe7]-α-MSH
T2 - A superpotent melanotropin with prolonged action on vertebrate chromatophores
AU - Hadley, Mac E.
AU - Mieyr, John H.
AU - Martin, Brent E.
AU - Ana, Ana M.
AU - Hruby, Victor J.
AU - Sawyer, Tomi K.
AU - Powers, Elaine A.
AU - Rao, K. Ranga
N1 - Funding Information:
nule translocations.I t is also possible,b ut probably moleculeistotally resistantoinactivation by serum unprecedentedth, at the analogue,a lthoughn o longer enzymes(Castruccietal., 1984). present, has in some way permanently induced a The melanotropin is now commerciallyavailable transductions ignal betweenr eceptora nd adenylate from a numberofpha~aceuti~aclompaniesa,sis the cyclaseW. hatevert he mechanismt,h ep rolongedb io-related fragment analogue, Ac-[Nle4, D-Phe’]-alogical activityo f them elanotropini s not restrictedto MSH,,,-NH,, which is also a superpotenatgonistin one or a few vertebrates pecies.I nstead, it appears severaml elanocytesystems(Sawyeretal., 1982)T. he that melanophoreso f at least some teleosts,a mphi-D-Phe’-containinganalogues houldproveusefulin a bians, reptiles,a nd mammals( Sawyere t al., 1980)a re variety of physiologicalstudies.For example,since similarly responsivet o the melanotropin analogue. follicular melanogenesisonly expresseduringhair The prolonged responseo f Lebistes erythrophores growth,thesemelanotropinanaloguesmightbe used and xanthophoresa s well as the prolongedr esponse to studypatternsofhair growthinmammalsthatare of ranid iridophorest o the analoguei ndicatet hat the responsivetomelanotropinsT.heshort-tailedweasel, unique interactionb etweent he melanotropina nd its for example,respondstoa-MSH (Rust and Meyer, targetc ell is not restrictedto melanophoresb ut is also 1968)andshould be a usefulmodelfor such studies. extendedt o other chromatophores. Acknowledgements-Paortf this work was supported by In addition to the demonstratedp rolongeda ctions Public Health Service grants MH-27257. MH-30626 and of [Nle4,D -Phe]-a-MSH directly on chromatophores AM-17420 and by grants PCM-883300. PCM-7707031 and in vitro (Sawyere t al., 1983)t, he in vivo actionso f the PCM-8110078 from the National Science Foundation. Dr. analoguea re probably furtherp rolongedb ecauset he A. M. Castrucci is a fellow of the Conselho National de
PY - 1985
Y1 - 1985
N2 - 1. 1. The in vitro and in vivo reponses of integumental chromatophores to α-MSH and a related analogue, [Nle4, D-Phe7]-α-MSH, were studied in a number of vertebrate species: the teleost, Lebistes reticulatus; the amphibians. Rana pipiens, R. catesbeiana, Xenopus laevis. Bufo alvarius, and B. cognatus; the lizard, Anolis carolinensis; the rattlesnake, Crotalus atrox. 2. 2. The α-melanotropin analogue was a superpotent agonist in the in vitro frog (R. pipiens, R. catesbeiana) and lizard (A. carolinensis) skin bioassays. 3. 3. In all species studied, the analogue exhibited ultraprolonged melanotropic activity, both in vitro and in vivo. 4. 4. This melanotropin and related analogues should prove useful in the study of numerous physiological processes, particularly when prolonged melanotropic activity is desired.
AB - 1. 1. The in vitro and in vivo reponses of integumental chromatophores to α-MSH and a related analogue, [Nle4, D-Phe7]-α-MSH, were studied in a number of vertebrate species: the teleost, Lebistes reticulatus; the amphibians. Rana pipiens, R. catesbeiana, Xenopus laevis. Bufo alvarius, and B. cognatus; the lizard, Anolis carolinensis; the rattlesnake, Crotalus atrox. 2. 2. The α-melanotropin analogue was a superpotent agonist in the in vitro frog (R. pipiens, R. catesbeiana) and lizard (A. carolinensis) skin bioassays. 3. 3. In all species studied, the analogue exhibited ultraprolonged melanotropic activity, both in vitro and in vivo. 4. 4. This melanotropin and related analogues should prove useful in the study of numerous physiological processes, particularly when prolonged melanotropic activity is desired.
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U2 - 10.1016/0300-9629(85)90259-2
DO - 10.1016/0300-9629(85)90259-2
M3 - Article
C2 - 2859941
AN - SCOPUS:0021816168
SN - 0300-9629
VL - 81
SP - 1
EP - 6
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
IS - 1
ER -