Nkx3.1 controls the DNA repair response in the mouse prostate

Hailan Zhang, Tian Zheng, Chee Wai Chua, Michael Shen, Edward P. Gelmann

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

BACKGROUND The human prostate tumor suppressor NKX3.1 mediates the DNA repair response and interacts with the androgen receptor to assure faithful completion of transcription thereby protecting against TMPRSS2-ERG gene fusion. To determine directly the effect of Nkx3.1 in vivo we studied the DNA repair response in prostates of mice with targeted deletion of Nkx3.1. METHODS Using both drug-induced DNA damage and γ-irradiation, we assayed expression of γ-histone 2AX at time points up to 24 hr after induction of DNA damage. RESULTS We demonstrated that expression of Nkx3.1 influenced both the timing and magnitude of the DNA damage response in the prostate. CONCLUSIONS Nkx3.1 affects the DNA damage response in the murine prostate and is haploinsufficient for this phenotype.

Original languageEnglish (US)
Pages (from-to)402-408
Number of pages7
JournalProstate
Volume76
Issue number4
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Keywords

  • DNA repair
  • NKX3.1
  • haploinsufficiency
  • γhistone 2AX

ASJC Scopus subject areas

  • Oncology
  • Urology

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