TY - JOUR
T1 - Nitroxyl affords thiol-sensitive myocardial protective effects akin to early preconditioning
AU - Pagliaro, Pasquale
AU - Mancardi, Daniele
AU - Rastaldo, Raffaella
AU - Penna, Claudia
AU - Gattullo, Donatella
AU - Miranda, Katrina M.
AU - Feelisch, Martin
AU - Wink, David A.
AU - Kass, David A.
AU - Paolocci, Nazareno
N1 - Funding Information:
We thank Prof. Fabio Di Lisa and Prof. Giuseppe Poli for helpful advice and suggestions. The authors are also grateful to Dr. Myron L. Weisfeldt for critically reviewing the manuscript and to Drs. Jon M. Fukuto and Joseph E. Saavedra for providing us with AS and DEA/NO. This study was supported by Compagnia di San Paolo, University of Turin and MIUR [(Cofin), PP] and by a Beginning Grant-in-Aid (0265435U) by the American Heart Association (NP).
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Nitric oxide (NO) donors mimic the early phase of ischemic preconditioning (IPC). The effects of nitroxyl (HNO/NO-), the one-electron reduction product of NO, on ischemia/reperfusion (I/R) injury are unknown. Here we investigated whether HNO/NO-, produced by decomposition of Angeli's salt (AS; Na2N2O3), has a cardioprotective effect in isolated perfused rat hearts. Effects were examined after intracoronary perfusion (19 min) of either AS (1 μM), the NO donor diethylamine/NO (DEA/NO, 0.5 μM), vehicle (100 nM NaOH) or buffer, followed by global ischemia (30 min) and reperfusion (30 min or 120 min in a subset of hearts). IPC was induced by three cycles of 3 min ischemia followed by 10 min reperfusion prior to I/R. The extent of I/R injury under each intervention was assessed by changes in myocardial contractility as well as lactate dehydrogenase (LDH) release and infarct size. Postischemic contractility, as indexed by developed pressure and dP/dtmax, was similarly improved with IPC and pre-exposure to AS, as opposed to control or DEA/NO-treated hearts. Infarct size and LDH release were also significantly reduced in IPC and AS groups, whereas DEA/NO was less effective in limiting necrosis. Co-infusion in the triggering phase of AS and the nitroxyl scavenger, N-acetyl-L-cysteine (4 mM) completely reversed the beneficial effects of AS, both at 30 and 120 min reperfusion. Our data show that HNO/NO- affords myocardial protection to a degree similar to IPC and greater than NO, suggesting that reactive nitrogen oxide species are not only necessary but also sufficient to trigger myocardial protection against reperfusion through species-dependent, pro-oxidative, and/or nitrosative stress-related mechanisms.
AB - Nitric oxide (NO) donors mimic the early phase of ischemic preconditioning (IPC). The effects of nitroxyl (HNO/NO-), the one-electron reduction product of NO, on ischemia/reperfusion (I/R) injury are unknown. Here we investigated whether HNO/NO-, produced by decomposition of Angeli's salt (AS; Na2N2O3), has a cardioprotective effect in isolated perfused rat hearts. Effects were examined after intracoronary perfusion (19 min) of either AS (1 μM), the NO donor diethylamine/NO (DEA/NO, 0.5 μM), vehicle (100 nM NaOH) or buffer, followed by global ischemia (30 min) and reperfusion (30 min or 120 min in a subset of hearts). IPC was induced by three cycles of 3 min ischemia followed by 10 min reperfusion prior to I/R. The extent of I/R injury under each intervention was assessed by changes in myocardial contractility as well as lactate dehydrogenase (LDH) release and infarct size. Postischemic contractility, as indexed by developed pressure and dP/dtmax, was similarly improved with IPC and pre-exposure to AS, as opposed to control or DEA/NO-treated hearts. Infarct size and LDH release were also significantly reduced in IPC and AS groups, whereas DEA/NO was less effective in limiting necrosis. Co-infusion in the triggering phase of AS and the nitroxyl scavenger, N-acetyl-L-cysteine (4 mM) completely reversed the beneficial effects of AS, both at 30 and 120 min reperfusion. Our data show that HNO/NO- affords myocardial protection to a degree similar to IPC and greater than NO, suggesting that reactive nitrogen oxide species are not only necessary but also sufficient to trigger myocardial protection against reperfusion through species-dependent, pro-oxidative, and/or nitrosative stress-related mechanisms.
KW - Angeli's salt
KW - Diethylamine/NO complex
KW - Free radicals
KW - Ischemia/reperfusion
KW - Myocardial necrosis
KW - N-acetyl-L-cysteine
KW - Nitric oxide
KW - Nitroxyl
KW - Preconditioning
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U2 - 10.1016/S0891-5849(02)01179-6
DO - 10.1016/S0891-5849(02)01179-6
M3 - Article
C2 - 12498977
AN - SCOPUS:12244282420
SN - 0891-5849
VL - 34
SP - 33
EP - 43
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 1
ER -