NHE8 plays important roles in gastric mucosal protection

Hua Xu, Jing Li, Huacong Chen, Chunhui Wang, Fayez K. Ghishan

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Sodium/hydrogen exchanger (NHE) 8 is an apically expressed membrane protein in the intestinal epithelial cells. It plays important roles in sodium absorption and bicarbonate secretion in the intestine. Although NHE8 mRNA has been detected in the stomach, the precise location and physiological role of NHE8 in the gastric glands remain unclear. In the current study, we successfully detected the expression of NHE8 in the glandular region of the stomach by Western blotting and located NHE8 protein at the apical membrane in the surface mucous cells by a confocal microscopic method. We also identified the expression of down regulated-in-adenoma (DRA) in the surface mucous cells in the stomach. Using NHE8-/- mice, we found that NHE8 plays little or no role in basal gastric acid production, yet NHE8-/- mice have reduced gastric mucosal surface pH and higher incidence of developing gastric ulcer. DRA expression was reduced significantly in the stomach in NHE8-/- mice. The propensity for gastric ulcer, reduced mucosal surface pH, and low DRA expression suggest that NHE8 is indirectly involved in gastric bicarbonate secretion and gastric mucosal protection.

Original languageEnglish (US)
Pages (from-to)G257-G261
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number3
StatePublished - Feb 1 2013


  • Down regulated-in-adenoma
  • Sodium/hydrogen exchanger 8

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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