TY - JOUR
T1 - New DNA polymorphism
T2 - Evidence for a low salt, left-handed form of poly(dG-m5dC)
AU - Feuerstein, Burt G.
AU - Marton, Laurence J.
AU - Keniry, Max A.
AU - Wade, Donald L.
AU - Shafer, Richard H.
N1 - Funding Information:
We thank Professor J.T. Yang for access to his CD instruments, Dr. N. Pattablraman for many helpful discussions, and Dr. T.M. Jovin for providing a preprint of unpublished work. This work was supported by U.S. Public Health Service grants CA 27343 and CA 13525 awarded by the National Cancer Institute, DHHS.
PY - 1985/6/11
Y1 - 1985/6/11
N2 - Spectroscopic studies on solutions of poly(dG-m5 dC) over a wide range of salt concentration are presented. Low salt solutions ([Na+]) < 2 mM) of poly(dG-m5dC) produce circular dichroism (CD) spectra typical of the left-handed, Z form at high salt ([Na+] = 1.75 M). Solutions of poly(dG-m5dC) at intermediate salt concentrations, e.g., 142 mM, yield CD spectra characteristic of the right-handed, B conformation. 31P NMR spectra of the low salt form of poly(dG-m5dC) reveal two well separated peaks, split by 1.4 ppm, consistent with a dinucleotide repeat. Kinetic studies show that the transition from the low salt form to the right-handed B form is slow, as expected for a major conformational change. These results suggest that the Z conformation in poly(dG-m5 dC) can be stabilized at very low salt as well as at high salt
AB - Spectroscopic studies on solutions of poly(dG-m5 dC) over a wide range of salt concentration are presented. Low salt solutions ([Na+]) < 2 mM) of poly(dG-m5dC) produce circular dichroism (CD) spectra typical of the left-handed, Z form at high salt ([Na+] = 1.75 M). Solutions of poly(dG-m5dC) at intermediate salt concentrations, e.g., 142 mM, yield CD spectra characteristic of the right-handed, B conformation. 31P NMR spectra of the low salt form of poly(dG-m5dC) reveal two well separated peaks, split by 1.4 ppm, consistent with a dinucleotide repeat. Kinetic studies show that the transition from the low salt form to the right-handed B form is slow, as expected for a major conformational change. These results suggest that the Z conformation in poly(dG-m5 dC) can be stabilized at very low salt as well as at high salt
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U2 - 10.1093/nar/13.11.4133
DO - 10.1093/nar/13.11.4133
M3 - Article
C2 - 4011444
AN - SCOPUS:0022423414
SN - 0305-1048
VL - 13
SP - 4133
EP - 4141
JO - Nucleic acids research
JF - Nucleic acids research
IS - 11
ER -