New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi

C. Robertson McClung; John D. Phillips; Marc J. Orbach; Jay C. Dunlap

doi:10.1016/0147-5975(89)90052-2

New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi

C. Robertson McClung, John D. Phillips, Marc J. Orbach, Jay C. Dunlap

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We describe five new plasmid vectors derived from pBR322 that carry theNeurospora crassa β-tubulin gene conferring resistance to benomyl. The benomyl resistance gene has been modified to eliminate an internalEcoRI site to facilitate the cloning ofEcoRI restriction fragments. These plasmids allow rapid subcloning of fragments from one replicon to another without insert fragment purification due to the presence of different drug resistance markers (resistance to kanamycin, tetracycline, or chloramphenicol) carried on the plasmids. These vectors will allow rapid transformation ofN. crassa and other filamentous fungi to allow phenotypic characterization of subcloned fragments.

Original language	English (US)
Pages (from-to)	299-302
Number of pages	4
Journal	Experimental Mycology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0147-5975(89)90052-2
State	Published - Sep 1989

Keywords

Neurospora
benomyl
filamentous fungi
pBR322
plasmids
subcloning
transformation
vectors
β-tubulin

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1016/0147-5975(89)90052-2

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@article{43937040634b4af3b54479805aeb200e,

title = "New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi",

abstract = "We describe five new plasmid vectors derived from pBR322 that carry theNeurospora crassa β-tubulin gene conferring resistance to benomyl. The benomyl resistance gene has been modified to eliminate an internalEcoRI site to facilitate the cloning ofEcoRI restriction fragments. These plasmids allow rapid subcloning of fragments from one replicon to another without insert fragment purification due to the presence of different drug resistance markers (resistance to kanamycin, tetracycline, or chloramphenicol) carried on the plasmids. These vectors will allow rapid transformation ofN. crassa and other filamentous fungi to allow phenotypic characterization of subcloned fragments.",

keywords = "Neurospora, benomyl, filamentous fungi, pBR322, plasmids, subcloning, transformation, vectors, β-tubulin",

author = "McClung, {C. Robertson} and Phillips, {John D.} and Orbach, {Marc J.} and Dunlap, {Jay C.}",

note = "Funding Information: This work was supported by NSF Grant DMB 84-17810, NIH Grant GM34985 and Core Grant Support CA-23 108 (through the Norris Cotton Cancer Center at Dartmouth Medical School) to J.C.D.",

year = "1989",

month = sep,

doi = "10.1016/0147-5975(89)90052-2",

language = "English (US)",

volume = "13",

pages = "299--302",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi

AU - McClung, C. Robertson

AU - Phillips, John D.

AU - Orbach, Marc J.

AU - Dunlap, Jay C.

N1 - Funding Information: This work was supported by NSF Grant DMB 84-17810, NIH Grant GM34985 and Core Grant Support CA-23 108 (through the Norris Cotton Cancer Center at Dartmouth Medical School) to J.C.D.

PY - 1989/9

Y1 - 1989/9

N2 - We describe five new plasmid vectors derived from pBR322 that carry theNeurospora crassa β-tubulin gene conferring resistance to benomyl. The benomyl resistance gene has been modified to eliminate an internalEcoRI site to facilitate the cloning ofEcoRI restriction fragments. These plasmids allow rapid subcloning of fragments from one replicon to another without insert fragment purification due to the presence of different drug resistance markers (resistance to kanamycin, tetracycline, or chloramphenicol) carried on the plasmids. These vectors will allow rapid transformation ofN. crassa and other filamentous fungi to allow phenotypic characterization of subcloned fragments.

AB - We describe five new plasmid vectors derived from pBR322 that carry theNeurospora crassa β-tubulin gene conferring resistance to benomyl. The benomyl resistance gene has been modified to eliminate an internalEcoRI site to facilitate the cloning ofEcoRI restriction fragments. These plasmids allow rapid subcloning of fragments from one replicon to another without insert fragment purification due to the presence of different drug resistance markers (resistance to kanamycin, tetracycline, or chloramphenicol) carried on the plasmids. These vectors will allow rapid transformation ofN. crassa and other filamentous fungi to allow phenotypic characterization of subcloned fragments.

KW - Neurospora

KW - benomyl

KW - filamentous fungi

KW - pBR322

KW - plasmids

KW - subcloning

KW - transformation

KW - vectors

KW - β-tubulin

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U2 - 10.1016/0147-5975(89)90052-2

DO - 10.1016/0147-5975(89)90052-2

M3 - Article

AN - SCOPUS:0001900838

SN - 1087-1845

VL - 13

SP - 299

EP - 302

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

IS - 3

ER -

New cloning vectors using benomyl resistance as a dominant marker for selection inNeurospora crassa and in other filamentous fungi

Abstract

Keywords

ASJC Scopus subject areas

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