Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum

E. W. Uhl; R. M. O'Connor; L. E. Perryman; M. W. Riggs

Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum

E. W. Uhl, R. M. O'Connor, L. E. Perryman, M. W. Riggs

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Isolates of Cryptosporidium parvum from New York, Florida, Brazil, Mexico, and Peru were examined for the presence of two sporozoite surface epitopes originally identified in an Iowa isolate by neutralizing monoclonal antibodies (MAbs) 18.44 and 17.41. Immunofluorescence microscopy and immunoblotting demonstrated the presence of both epitopes on all isolates. Incubation of DEAE-cellulose-purified sporozoites of the New York, Florida, Brazil, and Mexico isolates with MAb 18.44 or 17.41 significantly neutralized their infectivity for 4- to 6-day-old BALB/c mice. The results indicate that two neutralization-sensitive epitopes are conserved on geographically diverse C. parvum isolates.

Original language	English (US)
Pages (from-to)	1703-1706
Number of pages	4
Journal	Infection and Immunity
Volume	60
Issue number	4
State	Published - 1992

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

Cite this

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title = "Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum",

abstract = "Isolates of Cryptosporidium parvum from New York, Florida, Brazil, Mexico, and Peru were examined for the presence of two sporozoite surface epitopes originally identified in an Iowa isolate by neutralizing monoclonal antibodies (MAbs) 18.44 and 17.41. Immunofluorescence microscopy and immunoblotting demonstrated the presence of both epitopes on all isolates. Incubation of DEAE-cellulose-purified sporozoites of the New York, Florida, Brazil, and Mexico isolates with MAb 18.44 or 17.41 significantly neutralized their infectivity for 4- to 6-day-old BALB/c mice. The results indicate that two neutralization-sensitive epitopes are conserved on geographically diverse C. parvum isolates.",

author = "Uhl, {E. W.} and O'Connor, {R. M.} and Perryman, {L. E.} and Riggs, {M. W.}",

year = "1992",

language = "English (US)",

volume = "60",

pages = "1703--1706",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum

AU - Uhl, E. W.

AU - O'Connor, R. M.

AU - Perryman, L. E.

AU - Riggs, M. W.

PY - 1992

Y1 - 1992

N2 - Isolates of Cryptosporidium parvum from New York, Florida, Brazil, Mexico, and Peru were examined for the presence of two sporozoite surface epitopes originally identified in an Iowa isolate by neutralizing monoclonal antibodies (MAbs) 18.44 and 17.41. Immunofluorescence microscopy and immunoblotting demonstrated the presence of both epitopes on all isolates. Incubation of DEAE-cellulose-purified sporozoites of the New York, Florida, Brazil, and Mexico isolates with MAb 18.44 or 17.41 significantly neutralized their infectivity for 4- to 6-day-old BALB/c mice. The results indicate that two neutralization-sensitive epitopes are conserved on geographically diverse C. parvum isolates.

AB - Isolates of Cryptosporidium parvum from New York, Florida, Brazil, Mexico, and Peru were examined for the presence of two sporozoite surface epitopes originally identified in an Iowa isolate by neutralizing monoclonal antibodies (MAbs) 18.44 and 17.41. Immunofluorescence microscopy and immunoblotting demonstrated the presence of both epitopes on all isolates. Incubation of DEAE-cellulose-purified sporozoites of the New York, Florida, Brazil, and Mexico isolates with MAb 18.44 or 17.41 significantly neutralized their infectivity for 4- to 6-day-old BALB/c mice. The results indicate that two neutralization-sensitive epitopes are conserved on geographically diverse C. parvum isolates.

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M3 - Comment/debate

C2 - 1372299

AN - SCOPUS:0026546483

SN - 0019-9567

VL - 60

SP - 1703

EP - 1706

JO - Infection and Immunity

JF - Infection and Immunity

IS - 4

ER -

Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum

Abstract

ASJC Scopus subject areas

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