Neurotensin elevates cytosolic calcium in small cell lung cancer cells

Julie Staley; Gary Fiskum; Thomas P. Davis; Terry W. Moody

doi:10.1016/0196-9781(89)90015-6

Neurotensin elevates cytosolic calcium in small cell lung cancer cells

Julie Staley, Gary Fiskum, Thomas P. Davis, Terry W. Moody

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The ability of neurotensin (NT) to elevate cytosolic Ca²⁺ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca²⁺ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca²⁺ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca²⁺ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca²⁺ from intracellular stores. These data indicate that SCLC NT receptors may use Ca²⁺ as a second messenger.

Original language	English (US)
Pages (from-to)	1217-1221
Number of pages	5
Journal	Peptides
Volume	10
Issue number	6
DOIs	https://doi.org/10.1016/0196-9781(89)90015-6
State	Published - 1989

Keywords

Cytosolic calcium
Neurotensin
Small cell lung cancer

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(89)90015-6

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title = "Neurotensin elevates cytosolic calcium in small cell lung cancer cells",

abstract = "The ability of neurotensin (NT) to elevate cytosolic Ca2+ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca2+ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca2+ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca2+ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca2+ from intracellular stores. These data indicate that SCLC NT receptors may use Ca2+ as a second messenger.",

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T1 - Neurotensin elevates cytosolic calcium in small cell lung cancer cells

AU - Staley, Julie

AU - Fiskum, Gary

AU - Davis, Thomas P.

AU - Moody, Terry W.

PY - 1989

Y1 - 1989

N2 - The ability of neurotensin (NT) to elevate cytosolic Ca2+ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca2+ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca2+ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca2+ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca2+ from intracellular stores. These data indicate that SCLC NT receptors may use Ca2+ as a second messenger.

AB - The ability of neurotensin (NT) to elevate cytosolic Ca2+ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca2+ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca2+ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca2+ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca2+ from intracellular stores. These data indicate that SCLC NT receptors may use Ca2+ as a second messenger.

KW - Cytosolic calcium

KW - Neurotensin

KW - Small cell lung cancer

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Neurotensin elevates cytosolic calcium in small cell lung cancer cells

Abstract

Keywords

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