Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada

Louis Simard; Guy Bélair; S. Patricia Stock; Hervé Mauléon; Julie Dionne

doi:10.1163/156854107781352070

Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada

Louis Simard, Guy Bélair, S. Patricia Stock, Hervé Mauléon, Julie Dionne

Entomology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A survey of entomopathogenic nematodes from 38 golf courses was conducted in different climatic areas of Ontario and Québec provinces, Canada, during autumn 2002 and 2003. Soil samples were collected on golf course greens, fairways and roughs. Entomopathogenic nematodes were isolated from soil using wax moth (Galleria mellonella) and carrot weevil (Listronotus oregonensis) larvae as bait. Of the 436 samples (218 sites), 11 contained entomopathogenic nematodes for this study. All entomopathogenic nematode-positive samples were isolated from roughs. Isolates were characterised using DNA satellite probes and 28S rDNA sequence data. Both methods confirmed the identities of nematode isolates as Steinernema carpocapsae, S. feltiae and S. kraussei.

Original language	English (US)
Pages (from-to)	325-332
Number of pages	8
Journal	Nematology
Volume	9
Issue number	3
DOIs	https://doi.org/10.1163/156854107781352070
State	Published - May 2007

Keywords

Molecular diagnosis
Steinernema carpocapsae
Steinernema feltiae
Steinernema kraussei
Survey
Turfgrass

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Agronomy and Crop Science

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10.1163/156854107781352070

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title = "Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada",

abstract = "A survey of entomopathogenic nematodes from 38 golf courses was conducted in different climatic areas of Ontario and Qu{\'e}bec provinces, Canada, during autumn 2002 and 2003. Soil samples were collected on golf course greens, fairways and roughs. Entomopathogenic nematodes were isolated from soil using wax moth (Galleria mellonella) and carrot weevil (Listronotus oregonensis) larvae as bait. Of the 436 samples (218 sites), 11 contained entomopathogenic nematodes for this study. All entomopathogenic nematode-positive samples were isolated from roughs. Isolates were characterised using DNA satellite probes and 28S rDNA sequence data. Both methods confirmed the identities of nematode isolates as Steinernema carpocapsae, S. feltiae and S. kraussei.",

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AU - Simard, Louis

AU - Bélair, Guy

AU - Stock, S. Patricia

AU - Mauléon, Hervé

AU - Dionne, Julie

PY - 2007/5

Y1 - 2007/5

N2 - A survey of entomopathogenic nematodes from 38 golf courses was conducted in different climatic areas of Ontario and Québec provinces, Canada, during autumn 2002 and 2003. Soil samples were collected on golf course greens, fairways and roughs. Entomopathogenic nematodes were isolated from soil using wax moth (Galleria mellonella) and carrot weevil (Listronotus oregonensis) larvae as bait. Of the 436 samples (218 sites), 11 contained entomopathogenic nematodes for this study. All entomopathogenic nematode-positive samples were isolated from roughs. Isolates were characterised using DNA satellite probes and 28S rDNA sequence data. Both methods confirmed the identities of nematode isolates as Steinernema carpocapsae, S. feltiae and S. kraussei.

AB - A survey of entomopathogenic nematodes from 38 golf courses was conducted in different climatic areas of Ontario and Québec provinces, Canada, during autumn 2002 and 2003. Soil samples were collected on golf course greens, fairways and roughs. Entomopathogenic nematodes were isolated from soil using wax moth (Galleria mellonella) and carrot weevil (Listronotus oregonensis) larvae as bait. Of the 436 samples (218 sites), 11 contained entomopathogenic nematodes for this study. All entomopathogenic nematode-positive samples were isolated from roughs. Isolates were characterised using DNA satellite probes and 28S rDNA sequence data. Both methods confirmed the identities of nematode isolates as Steinernema carpocapsae, S. feltiae and S. kraussei.

KW - Molecular diagnosis

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KW - Steinernema feltiae

KW - Steinernema kraussei

KW - Survey

KW - Turfgrass

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Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada

Abstract

Keywords

ASJC Scopus subject areas

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