Native mass spectrometry characterization of intact nanodisc lipoprotein complexes

Michael T. Marty, Hao Zhang, Weidong Cui, Robert E. Blankenship, Michael L. Gross, Stephen G. Sligar

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

We describe here the analysis of nanodisc complexes by using native mass spectrometry (MS) to characterize their molecular weight (MW) and polydispersity. Nanodiscs are nanoscale lipid bilayers that offer a platform for solubilizing membrane proteins. Unlike detergent micelles, nanodiscs are native-like lipid bilayers that are well-defined and potentially monodisperse. Their mass spectra allow peak assignment based on differences in the mass of a single lipid per complex. Resultant masses agree closely with predicted values and demonstrate conclusively the narrow dispersity of lipid molecules in the nanodisc. Fragmentation with collisionally activated dissociation (CAD) or electron-capture dissociation (ECD) shows loss of a small number of lipids and eventual collapse of the nanodisc with release of the scaffold protein. These results provide a foundation for future studies utilizing nanodiscs as a platform for launching membrane proteins into the gas phase.

Original languageEnglish (US)
Pages (from-to)8957-8960
Number of pages4
JournalAnalytical Chemistry
Volume84
Issue number21
DOIs
StatePublished - Nov 6 2012
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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