Myricetin protects natural killer cells from arsenite induced DNA damage by attenuating oxidative stress and retaining poly(ADP-Ribose) polymerase 1 activity

Huijuan Ma, Xiaodong Song, Ping Huang, Weiwei Zhang, Xinyue Ling, Xiaoning Yang, Wenwei Wu, Huan Xu, Wei Wang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Environmental exposure to arsenite (As+3) is known to induce immunotoxicity. Natural killer (NK) cells are innate lymphoid cells act as professional killers of tumor cells. Our previous report indicated that 500 ppb As+3 drinking water exposure induced significant DNA damage in the NK cells of C57BL/6 mice. Myricetin is a plant-derived flavonoid known as a strong antioxidant. In this study, daily administration of myricetin at 20 mg/kg was found to alleviate the cell population decrease and DNA damage in the NK cells of BALB/c mice exposed to 500 and 1000 ppb As+3 via drinking water. Oxidative stress and poly(ADP-ribose) polymerase 1 (PARP-1) inhibition were induced by As+3 at 1 and 2 μM in isolated mouse NK cells in vitro, which were attenuated by 20 μM myricetin. The mitigatory effect of myricetin on the PARP-1 inhibition in NK cells treated with As+3 was also found to be the result of its prevention of the zinc loss induced by As+3 on PARP-1. Collectively, these results demonstrated, for the first time, that myricetin could protect NK cells from As+3 induced DNA through attenuating oxidative stress and retaining PARP-1 activity, indicating that myricetin may be utilized for the prevention of the immunotoxicity induced by As+3 in NK cells.

Original languageEnglish (US)
Article number503337
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume865
DOIs
StatePublished - May 2021

Keywords

  • DNA damage
  • Genotoxicity
  • Myricetin
  • NK cells
  • Oxidative stress
  • PARP-1

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

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