Muscle growth and satellite cell proliferative activity in obese (OB/OB) mice.

R. W. Purchas, D. R. Romsos, R. E. Allen, R. A. Merkel

    Research output: Contribution to journalArticlepeer-review

    13 Scopus citations


    Muscle growth of male obese (ob/ob) and lean mice at 2, 3, 5 and 8 wk were analyzed on the basis of weights of gastrocnemius, plantaris and soleus muscles from each hind leg. The carcasses (prepared by removing skin, viscera, head, feet and tail) were analyzed for fat content so that the effect of phenotype on the relationship between muscle weight and fat-free carcass weight could be assessed. For each age group the obese mice had less muscle relative to fat-free carcass weight than lean mice, with the difference being significant at 3 wk (P less than .05) and 8 wk (P less than .025). The proliferative activity of muscle satellite cells in 2- and 3-wk-old obese and lean mice was measured on isolated muscle fibers by autoradiography. Muscle fiber diameter and number of nuclei/unit length were unaffected by phenotype, but the proportion of muscle nuclei showing proliferative activity was lower (P less than .01) in obese than in lean mice at 2 wk (1.05 vs 1.93%, respectively) and 3 wk of age (.23 vs .59%, respectively). These results are consistent with the suggestion that muscle growth is limited by satellite-cell proliferative activity, although direct evidence for a cause and effect relationship is not provided.

    Original languageEnglish (US)
    Pages (from-to)644-651
    Number of pages8
    JournalJournal of animal science
    Issue number3
    StatePublished - Mar 1985

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics


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