Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin

Lin Mei; Henry I. Yamamura; William R. Roeske

doi:10.1016/0006-8993(88)91140-7

Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin

Lin Mei, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([³H]IP₁) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [³H]IP₁ accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/10⁶ cells to 42.4 fmol/10⁶ cells (P < 0.05) and 22.1 fmol/10⁶ cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC₅₀ values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [³H]IP₁ accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.

Original language	English (US)
Pages (from-to)	360-363
Number of pages	4
Journal	Brain Research
Volume	447
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(88)91140-7
State	Published - May 3 1988

Keywords

Human neuroblastoma
Muscarinic receptor
Pertussis toxin
Phosphatidylinositol hydrolysis
SH-SY5Y cell line

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(88)91140-7

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title = "Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin",

abstract = "Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/106 cells to 42.4 fmol/106 cells (P < 0.05) and 22.1 fmol/106 cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.",

keywords = "Human neuroblastoma, Muscarinic receptor, Pertussis toxin, Phosphatidylinositol hydrolysis, SH-SY5Y cell line",

author = "Lin Mei and Yamamura, {Henry I.} and Roeske, {William R.}",

note = "Funding Information: This work was supported in part by U.S. Public Health Service Grants HL-20984 and DK-36289. The authors would like to thank Ms. Pam Davis for secretarial service and Carol A. Haussler and Alfred C. Galiegos for cell culture assistance.",

year = "1988",

month = may,

day = "3",

doi = "10.1016/0006-8993(88)91140-7",

language = "English (US)",

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pages = "360--363",

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T1 - Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin

AU - Mei, Lin

AU - Yamamura, Henry I.

AU - Roeske, William R.

N1 - Funding Information: This work was supported in part by U.S. Public Health Service Grants HL-20984 and DK-36289. The authors would like to thank Ms. Pam Davis for secretarial service and Carol A. Haussler and Alfred C. Galiegos for cell culture assistance.

PY - 1988/5/3

Y1 - 1988/5/3

N2 - Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/106 cells to 42.4 fmol/106 cells (P < 0.05) and 22.1 fmol/106 cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.

AB - Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/106 cells to 42.4 fmol/106 cells (P < 0.05) and 22.1 fmol/106 cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.

KW - Human neuroblastoma

KW - Muscarinic receptor

KW - Pertussis toxin

KW - Phosphatidylinositol hydrolysis

KW - SH-SY5Y cell line

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Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin

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