Abstract
Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/106 cells to 42.4 fmol/106 cells (P < 0.05) and 22.1 fmol/106 cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.
Original language | English (US) |
---|---|
Pages (from-to) | 360-363 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 447 |
Issue number | 2 |
DOIs | |
State | Published - May 3 1988 |
Keywords
- Human neuroblastoma
- Muscarinic receptor
- Pertussis toxin
- Phosphatidylinositol hydrolysis
- SH-SY5Y cell line
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology