Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding

Diana Barritt; Henry I. Yamamura; William R. Roeske

doi:10.1016/0024-3205(82)90602-6

Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding

Diana Barritt
, Henry I. Yamamura
, William R. Roeske

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [³H]c is methyldioxolane ([³H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [³H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [³H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [³H](-) quinuclidinyl benzilate (QNB) was observed.

Original language	English (US)
Pages (from-to)	875-877
Number of pages	3
Journal	Life Sciences
Volume	30
Issue number	10
DOIs	https://doi.org/10.1016/0024-3205(82)90602-6
State	Published - Mar 8 1982

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(82)90602-6

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@article{db36a29539aa45d4b7885ae49f59cd3b,

title = "Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding",

abstract = "The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52\%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.",

author = "Diana Barritt and Yamamura, \{Henry I.\} and Roeske, \{William R.\}",

note = "Funding Information: The authors wish to acknowledge the excellent technical assistance of Linda Grunwald and secretarial assistance of Terry Austin. Portions of this research were supported by United States Public Health Service Grants MH-27257, MH-30626, and Program Project HL 20984. Henry I. Yamamura is a recipient of a USPHS RSDA, type II (MHO0095), from the NIMH. William R. Roeske is a recipient of a USPHS RCDA (HL-00776), from the NHLBI.",

year = "1982",

month = mar,

day = "8",

doi = "10.1016/0024-3205(82)90602-6",

language = "English (US)",

volume = "30",

pages = "875--877",

journal = "Life Sciences",

issn = "0024-3205",

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number = "10",

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TY - JOUR

T1 - Muscarinic receptor binding in the mouse heart

T2 - the selective modulatory effect of fluoride ion on agonist binding

AU - Barritt, Diana

AU - Yamamura, Henry I.

AU - Roeske, William R.

N1 - Funding Information: The authors wish to acknowledge the excellent technical assistance of Linda Grunwald and secretarial assistance of Terry Austin. Portions of this research were supported by United States Public Health Service Grants MH-27257, MH-30626, and Program Project HL 20984. Henry I. Yamamura is a recipient of a USPHS RSDA, type II (MHO0095), from the NIMH. William R. Roeske is a recipient of a USPHS RCDA (HL-00776), from the NHLBI.

PY - 1982/3/8

Y1 - 1982/3/8

N2 - The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.

AB - The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]c is methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2 nM, by 52%. Studies with several different ions demonstrated that the reduction in [3H]CD binding was a specific effect of fluoride. This fluoride modulation was selective for agonist binding, as no effect of fluoride on the binding of the muscarinic antagonist [3H](-) quinuclidinyl benzilate (QNB) was observed.

UR - https://www.scopus.com/pages/publications/0020472496

UR - https://www.scopus.com/pages/publications/0020472496#tab=citedBy

U2 - 10.1016/0024-3205(82)90602-6

DO - 10.1016/0024-3205(82)90602-6

M3 - Article

C2 - 7070200

AN - SCOPUS:0020472496

SN - 0024-3205

VL - 30

SP - 875

EP - 877

JO - Life Sciences

JF - Life Sciences

IS - 10

ER -

Muscarinic receptor binding in the mouse heart: the selective modulatory effect of fluoride ion on agonist binding

Abstract

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