Multispectral Mueller matrix imaging dark-field microscope for biological sample observation

Naooki Saito, Kenji Sato, Toru Fujii, Heather L. Durko, Goldie L. Goldstein, Alton H. Phillips, Joceline Dominguez-Cooks, Gabrielle V. Hutchens, Harrison T. Thurgood, Photini F. Rice, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Mueller polarimetric imaging in dark-field observation shows a contrast enhancement between healthy and cancerous human colon tissue in some reports. We have developed a Mueller-matrix microscope system that combines a dark-field polarization illuminator with an imaging polarimeter to measure the polarization characteristics of scattered light from human colon tissue samples. A multichannel light source permits the acquisition of multispectral Mueller matrices of the sample. The wavelength and polarization state selections are automated, as is the Mueller matrix measurement. The imaging polarimeter permits the system to perform fast, stable measurements. Calibration allows us to reduce the error associated with the illumination and imaging optics in the microscope system. Our system indicates a clear difference between the average Mueller matrix measurements of healthy and cancerous human colon tissue, which agrees well with previously reported results.

Original languageEnglish (US)
Title of host publicationLabel-Free Biomedical Imaging and Sensing, LBIS 2019
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510624221
DOIs
StatePublished - 2019
EventLabel-Free Biomedical Imaging and Sensing 2019, LBIS 2019 - San Francisco, United States
Duration: Feb 2 2019Feb 5 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10890
ISSN (Print)1605-7422

Conference

ConferenceLabel-Free Biomedical Imaging and Sensing 2019, LBIS 2019
Country/TerritoryUnited States
CitySan Francisco
Period2/2/192/5/19

Keywords

  • Dark-field illumination
  • Microscope
  • Mueller polarimetry
  • Multispectral imaging
  • Polarization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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