Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse.

P. H. Schreier, A. L. Bothwell, B. Mueller-Hill, D. Baltimore

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Abstract

To compare the structure of IgG2a alleles we have determined the complete DNA sequence of the constant region, coding sequence, and 3' untranslated region of a cDNA clone, pAB gamma 2a-1, which was derived from the C57BL/6 mouse strain (b allotype). This sequence was compared with the corresponding IgG2a DNA sequence of BALB/c origin (a allotype). The DNA sequences showed 10% differences, and the deduced protein sequences differed by about 15%. These differences were not evenly distributed: most differences were in the hinge region, the CH3 domain and the 3' untranslated region. It is evident that many alterations in the IgG2a alleles have occurred since the a and b haplotypes were separated--some of these changes were point mutations but some appear to have resulted from gene conversion of the IgG2ab allele by the IgG2bb allele.

Original languageEnglish (US)
Pages (from-to)4495-4499
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number7
DOIs
StatePublished - Jul 1981
Externally publishedYes

ASJC Scopus subject areas

  • General

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