Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

Gangjun Liu; Khanh Kieu; Frank W. Wise; Zhongping Chen

doi:10.1002/jbio.201000049

Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

Gangjun Liu, Khanh Kieu, Frank W. Wise, Zhongping Chen

Optical Sciences

Research output: Contribution to journal › Letter › peer-review

52 Scopus citations

Abstract

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

Original language	English (US)
Pages (from-to)	34-39
Number of pages	6
Journal	Journal of Biophotonics
Volume	4
Issue number	1-2
DOIs	https://doi.org/10.1002/jbio.201000049
State	Published - Jan 2011

Keywords

Fiber laser
Hand-held probe
Multiphoton processes
Nonlinear microscopy

ASJC Scopus subject areas

General Chemistry
General Materials Science
General Biochemistry, Genetics and Molecular Biology
General Engineering
General Physics and Astronomy

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10.1002/jbio.201000049

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title = "Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe",

abstract = "We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.",

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author = "Gangjun Liu and Khanh Kieu and Wise, {Frank W.} and Zhongping Chen",

year = "2011",

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AU - Liu, Gangjun

AU - Kieu, Khanh

AU - Wise, Frank W.

AU - Chen, Zhongping

PY - 2011/1

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N2 - We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

AB - We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

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KW - Multiphoton processes

KW - Nonlinear microscopy

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Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

Abstract

Keywords

ASJC Scopus subject areas

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