Multi-wavelength quantitative polarization and phase microscope

Xiaobo Tian, Xingzhou Tu, Kimiko Della Croce, Guang Yao, Haijiang Cai, Neal Brock, Stanley Pau, Rongguang Liang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


We introduce a snapshot multi-wavelength quantitative polarization and phase microscope (MQPPM) for measuring spectral dependent quantitative polarization and phase information. The system uniquely integrates a polarized light microscope and a snap-shot quantitative phase microscope in a single system, utilizing a novel full-Stokes camera operating in the red, green, and blue (RGB) spectrum. The linear retardance and fast axis orientation of a birefringent sample can be measured simultaneously in the visible spectra. Both theoretical analysis and experiments have been performed to demonstrate the capability of the proposed microscope. Data from liquid crystal and different biological samples are presented. We believe that MQPPM will be a useful tool in measuring quantitative polarization and phase information of live cells.

Original languageEnglish (US)
Pages (from-to)1638-1648
Number of pages11
JournalBiomedical Optics Express
Issue number4
StatePublished - Apr 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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